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Home >> Antibodies >> LKB1 Antibody

LKB1 Antibody (F50223)

  Catalog No Formulation Size Price (USD)  
Image F50223-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F50223-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Confocal immunofluorescent analysis of LKB1 antibody with ZR-75-1 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
LKB1 antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Western blot analysis of LKB1 antibody and Ramos lysate. Predicted molecular weight: 50~60 kDa
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Chicken, Mouse, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt Q15831
Applications Western blot : 1:1000
Flow Cytometry : 1:10-1:50
Immunofluorescence : 1:10-1:50
Limitations This LKB1 antibody is available for research use only.
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Description

LKB1 is a tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. [UniProt]

Application Notes

Titration of the LKB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 19-48 from the human protein was used as the immunogen for this LKB1 antibody.

Storage

Aliquot the LKB1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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