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Home >> Antibodies >> Lipocalin 2 Antibody (NGAL, LCN2)

Lipocalin 2 Antibody (NGAL, LCN2) (R31946)

  Catalog No Formulation Size Price (USD)  
Image R31946 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat spleen and 2) rat lung lysate with Lipocalin 2 antibody. Predicted/observed molecular weight: 22-25 kDa.
IHC testing of FFPE mouse spleen with Lipocalin 2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat spleen with Lipocalin 2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of frozen mouse spleen tissue with Lipocalin 2 antibody.
IHC testing of frozen mouse lung tissue with Lipocalin 2 antibody.
IHC testing of frozen rat spleen tissue with Lipocalin 2 antibody.
IHC testing of frozen rat lung tissue with Lipocalin 2 antibody.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt P11672
Localization Cytoplasmic, secreted
Applications Western blot : 0.1-0.5ug/ml
IHC (FFPE) : 0.5-1ug/ml
IHC (Frozen) : 0.5-1ug/ml
ELISA : 0.1-0.5ug/ml (mouse protein tested); request BSA-free format for coating
Limitations This Lipocalin 2 antibody is available for research use only.
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Description

Europhile gelatinase-associated lipocalin (NGAL) is a protein that in humans is encoded by the LCN2 gene. The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor.

Application Notes

Optimal dilution of the Lipocalin 2 antibody should be determined by the researcher.

Immunogen

Amino acids 21-200 of mouse Lipocalin 2 were used as the immunogen for the Lipocalin 2 antibody.

Storage

After reconstitution, the Lipocalin 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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