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Home >> Antibodies >> Lingo1 Antibody

Lingo1 Antibody (F54318)

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  Catalog No Formulation Size Price (USD)  
Image F54318-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54318-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human brain tissue with Lingo1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human brain tissue with Lingo1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human brain tissue with Lingo1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of mouse 1) brain and 2) cerebellum lysate with Lingo1 antibody. Expected molecular weight: 69-83 kDa depending on glycosylation level.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q9D1T0
Applications Western blot : 1:500-1:2000
Immunohistochemistry (FFPE) : 1:25
Limitations This Lingo1 antibody is available for research use only.
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Description

'Leucine-rich repeat and immunoglobulin domain-containing protein 1' is the functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation responsible for some inhibition of axonal regeneration by myelin-associated factors. LINGO1 is also an important negative regulator of oligodentrocyte differentiation and axonal myelination. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the Lingo1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 575-603 from the mouse protein was used as the immunogen for the Lingo1 Antibody.

Storage

Aliquot the Lingo1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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