LI Cadherin Antibody Recombinant Mouse MAb / Cadherin 17 [clone rCDH17/8514] (V5219)

Immunohistochemistry of LI Cadherin antibody in human colon tissue. The recombinant mouse mAb clone rCDH17/8514 demonstrates strong membranous HRP-DAB brown staining along the lateral borders of colonic epithelial cells, consistent with Cadherin 17 localization in intestinal epithelium. Staining highlights well-organized glandular crypt structures with preserved epithelial polarity, while surrounding stromal tissue shows minimal background signal. The negative control inset, using PBS in place of the primary antibody, shows no specific staining. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to incubation.

SDS-PAGE analysis of purified, BSA-free LI Cadherin antibody (clone rCDH17/8514) as confirmation of integrity and purity.

Western blot analysis of LI Cadherin antibody in human small intestine tissue lysate. The recombinant mouse mAb clone rCDH17/8514 detects a prominent band above 92 kDa, consistent with Cadherin 17. The predicted molecular weight of Cadherin 17 is approximately 92 kDa based on its amino acid sequence; however, the protein is heavily glycosylated, which commonly results in slower migration and an apparent higher molecular weight on SDS-PAGE. The observed band pattern aligns with expected glycosylated CDH17 expression in small intestinal epithelial tissue.