LI Cadherin Antibody Recombinant Mouse MAb / Cadherin 17 [clone rCDH17/8514] (V5219)

LI Cadherin Antibody Colon IHC. Immunohistochemistry staining of FFPE human colon tissue using LI Cadherin Antibody / CDH17 Antibody (recombinant mouse monoclonal clone rCDH17/8514) demonstrates strong membranous HRP-DAB brown staining along the lateral borders of colonic epithelial cells. The staining pattern is consistent with expression of Cadherin 17 (CDH17, LI-cadherin), a calcium-dependent cell adhesion molecule that contributes to epithelial organization, maintenance of tissue integrity, and regulation of cell-cell interactions within the gastrointestinal tract. Prominent membrane-associated staining highlights well-organized glandular crypt structures and preserved epithelial polarity, while surrounding stromal tissues exhibit minimal background reactivity. The inset image shows the negative control in which PBS was substituted for the primary antibody. HIER was performed by boiling tissue sections in pH 9, 10 mM Tris with 1 mM EDTA for 20 minutes followed by cooling prior to immunostaining.

SDS-PAGE analysis of purified, BSA-free LI Cadherin antibody (clone rCDH17/8514) as confirmation of integrity and purity.

LI Cadherin Antibody WB. Western blot analysis of human small intestine tissue lysate using LI Cadherin Antibody / CDH17 Antibody (recombinant mouse monoclonal clone rCDH17/8514) demonstrates a strong immunoreactive band at approximately 100-110 kDa, consistent with expression of Cadherin 17 (CDH17, LI-cadherin). CDH17 is a calcium-dependent cell adhesion molecule predominantly expressed in gastrointestinal epithelial tissues, where it contributes to epithelial organization, maintenance of tissue integrity, and regulation of cell-cell interactions. The observed molecular weight is higher than the predicted size of approximately 92 kDa, a finding commonly attributed to extensive glycosylation of the extracellular domain. Such post-translational modification frequently results in slower electrophoretic migration and an increased apparent molecular weight during western blot analysis. The observed banding pattern is consistent with expected glycosylated CDH17 expression in human small intestinal epithelium. Predicted molecular weight: approximately 92 kDa.