LGALS3 Antibody LGALS3/4792 | Galectin-3

	Catalog No	Formulation	Size	Price (USD)
	V9668-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	100 ug	559
Formulation
	V9668-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	20 ug	259
Formulation
	V9668SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation

LGALS3 Antibody by IHC in human thyroid carcinoma. Immunohistochemistry of FFPE human thyroid carcinoma tissue using LGALS3 Antibody LGALS3/4792. Brown immunoreactivity is predominantly cytoplasmic in tumor cells, with variable staining intensity across the glandular structures. Heat-induced epitope retrieval was performed by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes.

LGALS3 Monoclonal Antibody Purity by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free LGALS3 Antibody LGALS3/4792 under reducing (R) and non-reducing (NR) conditions, with 2 ug of antibody loaded per lane. The gel shows an intact antibody band under non-reducing conditions and heavy and light chains under reducing conditions.

LGALS3 Antibody Specificity by HuProt Protein Microarray. Analysis of a HuProt microarray containing more than 19,000 full-length human proteins using LGALS3 Antibody LGALS3/4792. The graph shows LGALS3 as the highest-ranking target based on antibody-binding Z-scores. The S-score represents the difference between the highest and second-highest Z-scores and provides a measure of relative binding specificity.

›

LGALS3 Antibody by IHC in human thyroid carcinoma. Immunohistochemistry of FFPE human thyroid carcinoma tissue using LGALS3 Antibody LGALS3/4792. Brown immunoreactivity is predominantly cytoplasmic in tumor cells, with variable staining intensity across the glandular structures. Heat-induced epitope retrieval was performed by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes.

LGALS3 Antibody by IHC in human thyroid carcinoma. Immunohistochemistry of FFPE human thyroid carcinoma tissue using LGALS3 Antibody LGALS3/4792. Brown cytoplasmic staining is visible throughout the tumor epithelium, highlighting the glandular architecture. Heat-induced epitope retrieval was performed by boiling sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes.

LGALS3 Monoclonal Antibody Purity by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free LGALS3 Antibody LGALS3/4792 under reducing (R) and non-reducing (NR) conditions, with 2 ug of antibody loaded per lane. The gel shows an intact antibody band under non-reducing conditions and heavy and light chains under reducing conditions.

LGALS3 Antibody Specificity by HuProt Protein Microarray. Analysis of a HuProt microarray containing more than 19,000 full-length human proteins using LGALS3 Antibody LGALS3/4792. The graph shows LGALS3 as the highest-ranking target based on antibody-binding Z-scores. The S-score represents the difference between the highest and second-highest Z-scores and provides a measure of relative binding specificity.

Species Reactivity	Human
Format	Purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG1, kappa
Clone Name	LGALS3/4792
Purity	Protein A/G affinity
UniProt	P17931
Localization	Cytoplasm
Applications	Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations	This LGALS3 Antibody LGALS3/4792 / Galectin-3 Monoclonal Antibody is available for research use only.
	Review this product on BioCompare and get a $20 Amazon gift card

Description

LGALS3 Antibody LGALS3/4792 / Galectin-3 Monoclonal Antibody recognizes galectin-3, a beta-galactoside-binding lectin encoded by the LGALS3 gene. Also known as Mac-2, CBP35, and IgE-binding protein, galectin-3 participates in cell adhesion, immune regulation, inflammation, and tissue remodeling. It belongs to the galectin family of carbohydrate-binding proteins, which recognize beta-galactoside-containing glycans on glycoproteins and glycolipids. Unlike many other galectins, galectin-3 contains an N-terminal region that supports oligomerization and a C-terminal carbohydrate recognition domain responsible for glycan binding.

Galectin-3 is distributed among several cellular compartments, including the cytoplasm, nucleus, cell surface, and extracellular environment. Its localization influences its biological activities. Intracellular galectin-3 participates in signaling pathways associated with cell survival, proliferation, and cellular stress responses. Extracellular galectin-3 can form multivalent complexes with glycosylated proteins, contributing to cell-surface receptor organization, adhesion, and communication between cells and their surrounding extracellular matrix.

In the immune system, galectin-3 is expressed by macrophages and several other immune cell populations. It contributes to inflammatory signaling, immune cell recruitment, and responses to tissue injury. Galectin-3 has also been investigated in fibrotic disorders, where its activities can influence fibroblast activation, extracellular matrix deposition, and tissue remodeling. These functions are relevant to cardiovascular, pulmonary, hepatic, and renal fibrosis research, although the protein's contributions depend on the cellular environment and disease context.

Galectin-3 expression has been investigated in numerous malignancies, including thyroid, breast, colorectal, and prostate cancers. Depending on cellular context, the protein may influence tumor cell survival, adhesion, migration, and interactions with the tumor microenvironment. Changes in its expression and subcellular distribution have been associated with malignant transformation and tumor progression. However, these relationships vary among cancer types and experimental settings, and expression patterns must be interpreted within the appropriate biological and pathological context.

The diverse functions of galectin-3 reflect its ability to participate in intracellular regulatory mechanisms and extracellular glycan-dependent interactions. Its involvement in inflammation, fibrosis, and cellular communication makes it an important target for investigating tissue organization and disease-associated remodeling. NSJ Bioreagents offers this LGALS3 Antibody for research examining glycan recognition, cellular adhesion, inflammatory regulation, and the molecular mechanisms underlying extracellular matrix interactions.

Explore our Galectin-3 Antibody LGALS3/6583 for additional research tools targeting LGALS3, with Western blot, immunohistochemistry, and HuProt protein microarray validation.

Application Notes

Optimal dilution of the LGALS3 Antibody LGALS3/4792 / Galectin-3 Monoclonal Antibody should be determined by the researcher.

Immunogen

A portion of amino acids 150-200 from the human protein was used as the immunogen for the LGALS3 antibody.

Storage

Aliquot the LGALS3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Alternate Names

Galectin-3 antibody, Galectin 3 antibody, Mac-2 antibody, Galactoside-binding lectin 3 antibody, IgE-binding protein antibody

Related Products

1. View all Galectin 3 antibodies

LGALS3 Antibody LGALS3/4792 / Galectin-3 Monoclonal Antibody [clone LGALS3/4792] (V9668)

Description

Application Notes

Immunogen

Storage

Alternate Names

Related Products

Area of Research

Product Type

Protocols

Contact Us

NSJ Bioreagents