LFA-2 Antibody / Microarray Specificity Validated / CD2 [clone LFA2/7100] (V4059)

Immunohistochemistry analysis of LFA-2 antibody (clone LFA2/7100) in human colon tissue. Formalin-fixed, paraffin-embedded colon demonstrates membranous HRP-DAB brown staining in scattered lymphoid cells within the lamina propria, consistent with CD2 expression on resident T lymphocytes. Colonic epithelial cells lining the glands are largely negative, highlighting the expected immune cell-restricted expression pattern. Hematoxylin counterstain delineates nuclear morphology and glandular architecture. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 20 minutes followed by cooling prior to staining.

IHC staining of FFPE human tonsil tissue with LFA-2 antibody (clone LFA2/7100). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using LFA-2 antibody (clone LFA2/7100). These results demonstrate the foremost specificity of the LFA2/7100 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free LFA-2 antibody (clone LFA2/7100) as confirmation of integrity and purity.