LFA-1 Antibody / CD18 / ITGB2 (R31562)

Western blot testing of LFA-1 antibody and Lane 1: Jurkat; 2: CCRF-CEM lysate; Predicted/Observed molecular weight: 85~95 kDa depending on glycosylation level.

Western blot testing of LFA-1 antibody and recombinant human partial protein (0.5ng/lane).

IHC-P: LFA-1 antibody testing of human lung cancer tissue. HIER: steamed with pH6 citrate buffer.

Immunofluorescent staining of human THP1 cells with LFA-1 antibody (green) at 2ug/ml and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of human tonsil tissue with LFA-1 antibody (green) at 1ug/ml and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry testing of human ThP-1 cells with LFA-1 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LFA-1 antibody.

Flow cytometry testing of human Raji cells with LFA-1 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LFA-1 antibody.

Flow cytometry testing of human U-2 OS cells with LFA-1 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LFA-1 antibody.