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Home >> Antibodies >> LEPR Antibody / Leptin Receptor / Ob-R

LEPR Antibody / Leptin Receptor / Ob-R [clone LEPR/4304] (V9667)

  Catalog No Formulation Size Price (USD)  
Image V9667-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V9667-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V9667SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human brain tissue with LEPR antibody (clone LEPR/4304). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human brain tissue with LEPR antibody (clone LEPR/4304). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free LEPR antibody (clone LEPR/4304) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using LEPR antibody (clone LEPR/4304). These results demonstrate the foremost specificity of the LEPR/4304 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name LEPR/4304
Purity Protein A/G affinity
UniProt P48357
Localization Secreted, Cell surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This LEPR antibody is available for research use only.
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Description

Although there is substantial evidence that body weight is physiologically regulated, the molecular basis of obesity is unknown. Five single-gene mutations in mice that result in an obese phenotype have been identified. The first such recessive obesity mutation, the obese mutation (Ob), was identified in 1950. Mutation of Ob results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. It has been postulated that the Ob gene product may function as a component of a signaling pathway in adipose tissue that functions to regulate body fat depot size. The cloning and sequence analysis of the mouse Ob gene and its human homolog has recently been described. Ob encodes an adipose tissue-specific mRNA with a highly conserved 167 amino acid open reading frame. The predicted amino acid sequence is 84% identical between human and mouse and has the features of a secreted protein. A nonsense mutation in codon 105 has been found in the original congenic C57BL/6J Ob/Ob mouse strain. The Ob gene encodes the protein leptin. The leptin receptor, designated Ob-R, has been shown to be a single membrane-spanning receptor that most resembles the gp130 signal transducing component of the IL-6, G-CSF and LIF receptor. Ob-R mRNA is expressed in the choroid plexus and hypothalamus.

Application Notes

Optimal dilution of the LEPR antibody should be determined by the researcher.

Immunogen

A portion of amino acids 335-425 was used as the immunogen for the LEPR antibody.

Storage

Aliquot the LEPR antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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