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Home >> Antibodies >> LDHD Antibody / D-lactate dehydrogenase

LDHD Antibody / D-lactate dehydrogenase (RQ8565)

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  Catalog No Formulation Size Price (USD)  
Image RQ8565 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Flow cytometry testing of fixed and permeabilized human K562 cells with LDHD antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LDHD antibody.
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Flow cytometry testing of fixed and permeabilized human K562 cells with LDHD antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LDHD antibody.
Western blot testing of 1) human HCCP, 2) rat kidney and 3) mouse kidney tissue lysate with LDHD antibody. Predicted molecular weight ~55 kDa, ~52 kDa (two isoforms).
Availability 1-3 days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q86WU2
Applications Western Blot : 0.5-1ug/ml
Flow Cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml
Limitations This LDHD antibody is available for research use only.
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Description

Two naturally occurring forms of lactate dehydrogenase with similar but unique substrate specificities have been isolated in lower organisms including invertebrates, fungi, and prokaryotes. These dehydrogenase enzymes are L-lactate dehydrogenase and D-lactate dehydrogenase (LDHD) that are specific to the L and D isomers of lactate, respectively (PMID: 12127981). In lactic acid bacteria, LDHD plays a key role in anaerobic energy metabolism (PMID: 497162). Despite the identification of D-lactate and other D-2-hydroxyacids in prokaryotes, and the obvious connections and similarities to vertebrate metabolic pathways, very few mammalian D-2-hydroxyacid dehydrogenases have been found. LDHD has 2 isoforms with the molecular weight of 52 and 54kDa, and can be detected as 45-54 kDa.

Application Notes

Optimal dilution of the LDHD antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (amino acids R56-Q498) was used as the immunogen for the LDHD antibody.

Storage

After reconstitution, the LDHD antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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