LCN2 Antibody / NGAL Antibody (R32053)

LCN2 Antibody staining of mouse splenic cell populations by IHC. Immunohistochemistry of FFPE mouse spleen sections using the LCN2 Antibody at 2 ug/ml. Scattered brown-positive cells are visible within the splenic tissue against a predominantly blue hematoxylin-counterstained background. Positive staining is relatively focal rather than broadly distributed throughout the section. Heat-mediated antigen retrieval was performed in pH 8 EDTA before overnight primary antibody incubation at 4oC. The localized cellular staining demonstrates tissue detection of Lipocalin 2 with this NGAL Antibody.

LCN2 Antibody detection of positive cells in mouse spleen by IHC. Immunohistochemistry of FFPE mouse spleen sections using the LCN2 Antibody. At higher magnification, distinct brown staining is visible in a population of cells surrounded by densely packed blue-counterstained splenic cells. The positive cells display predominantly cytoplasmic staining and are clearly distinguishable from the surrounding negative population. Heat-induced epitope retrieval was performed by boiling sections in pH 6 citrate buffer for 20 minutes followed by cooling before testing. The staining demonstrates cellular detection of Lipocalin 2 with this NGAL Antibody.

LCN2 Antibody staining of focal cellular regions in mouse spleen by IHC. Immunohistochemistry of FFPE mouse spleen sections using the LCN2 Antibody. Strong brown staining is concentrated within discrete groups of cells, while much of the surrounding densely cellular splenic tissue remains comparatively negative. The localized staining provides a second view of the heterogeneous distribution of LCN2-positive cells within mouse spleen. Heat-induced epitope retrieval was performed by boiling sections in pH 6 citrate buffer for 20 minutes followed by cooling before testing with this NGAL Antibody.

LCN2 Antibody staining of scattered cells in rat spleen by IHC. Immunohistochemistry of FFPE rat spleen sections using the LCN2 Antibody at 2 ug/ml. Scattered brown-positive cells are visible within the blue-counterstained splenic tissue, with positive cells occurring individually and in small localized groups. The surrounding tissue shows substantially less chromogenic signal. Heat-mediated antigen retrieval was performed in pH 8 EDTA before overnight primary antibody incubation at 4oC. The focal cellular staining demonstrates tissue detection of Lipocalin 2 with this NGAL Antibody.

LCN2 Antibody detection of strongly positive cells in rat spleen by IHC. Immunohistochemistry of FFPE rat spleen sections using the LCN2 Antibody at 2 ug/ml. Strong brown cytoplasmic staining is concentrated within a discrete population of cells surrounded by predominantly blue-counterstained splenic cells. The marked contrast between positive and negative populations highlights the heterogeneous cellular distribution of LCN2 within the tissue. Heat-mediated antigen retrieval was performed in pH 8 EDTA before overnight primary antibody incubation at 4oC. The staining demonstrates detection of Lipocalin 2 with this NGAL Antibody.

LCN2 Antibody localization within rat splenic tissue by IHC. Immunohistochemistry of FFPE rat spleen sections using the LCN2 Antibody at 2 ug/ml. Brown-positive cells are distributed primarily within portions of the more densely cellular splenic tissue, while the large pale region and surrounding areas show comparatively limited staining. This lower-magnification view illustrates the nonuniform distribution of LCN2-positive cells across the section. Heat-mediated antigen retrieval was performed in pH 8 EDTA before overnight primary antibody incubation at 4oC. The staining demonstrates tissue recognition with this NGAL Antibody.

LCN2 Antibody detection in rat and mouse lung by WB. Western blot testing of rat lung (lane 1) and mouse lung (lane 2) tissue lysates with the LCN2 Antibody at 0.5 ug/ml under reducing conditions. Thirty micrograms of protein were loaded per lane. A strong predominant band is detected at approximately 22-23 kDa in both samples, consistent with the expected migration of Lipocalin 2. The rat sample also shows a weaker lower-molecular-weight signal, while the major target band is clearly resolved in both species. These results demonstrate detection of LCN2 in rat and mouse lung with this NGAL Antibody.