LCK Antibody / T-Cell Signaling Kinase Antibody (R31894)

LCK Antibody Jurkat, Thymus and Spleen Western Blot. Western blot analysis of human Jurkat cells (lane 1), rat thymus (lane 2), rat spleen (lane 3), mouse thymus (lane 4), and mouse spleen (lane 5) using LCK Antibody / T-Cell Signaling Kinase Antibody demonstrates distinct bands at approximately 58 kDa, consistent with the expected molecular weight of LCK. LCK is a SRC family tyrosine kinase that serves as a critical initiator of T-cell receptor signaling, regulating pathways involved in lymphocyte activation, differentiation, proliferation, and adaptive immune responses. Detection of LCK in Jurkat cells and lymphoid tissues including thymus and spleen is consistent with its established role in T-cell development and immune signaling. The observed expression pattern supports the utility of LCK as a marker of T-cell-associated signal transduction and adaptive immune function.

LCK Antibody Human Tonsil IHC. Immunohistochemical staining of FFPE human tonsil tissue using LCK Antibody demonstrates prominent membranous and cytoplasmic HRP-DAB brown staining within lymphocyte-rich regions. The staining pattern is consistent with expression of LCK, a SRC family tyrosine kinase that functions as a critical initiator of T-cell receptor signaling and adaptive immune activation. LCK-mediated signaling regulates lymphocyte activation, proliferation, differentiation, and immune communication, making it an essential component of T-cell biology. The strong staining observed in tonsillar lymphoid populations supports the established role of LCK in immune signaling pathways and adaptive immune function. HIER: boil paraffin sections in pH 6, 10 mM citrate buffer for 20 minutes and allow to cool prior to staining.

LCK Antibody Mouse Lymph Node IHC. Immunohistochemical staining of FFPE mouse lymph node tissue using LCK Antibody / T-Cell Signaling Kinase Antibody demonstrates prominent membranous and cytoplasmic HRP-DAB brown staining within dense lymphoid cell populations. The staining pattern is consistent with expression of LCK, a SRC family tyrosine kinase that serves as a critical initiator of T-cell receptor signaling and downstream adaptive immune responses. LCK regulates signaling pathways involved in lymphocyte activation, proliferation, differentiation, and immune communication. The strong signal observed within lymph node-associated lymphocytes supports the established role of LCK in T-cell biology and immune system regulation. HIER: boil paraffin sections in pH 6, 10 mM citrate buffer for 20 minutes and allow to cool prior to staining.

LCK Antibody Rat Lymph Node IHC. Immunohistochemical staining of FFPE rat lymph node tissue using LCK Antibody / T-Cell Signaling Kinase Antibody demonstrates strong membranous and cytoplasmic HRP-DAB brown staining throughout lymphocyte-rich regions. The staining pattern is consistent with expression of LCK, a SRC family tyrosine kinase that functions as a critical initiator of T-cell receptor signaling and adaptive immune activation. LCK-mediated phosphorylation events regulate pathways involved in lymphocyte activation, proliferation, differentiation, and immune communication. The prominent staining observed within lymphoid cell populations supports the established role of LCK in T-cell biology, immune surveillance, and adaptive immune responses. HIER: boil paraffin sections in pH 6, 10 mM citrate buffer for 20 minutes and allow to cool prior to staining.

LCK Antibody Mouse Spleen IHC. Immunohistochemical staining of frozen mouse spleen tissue using LCK Antibody / T-Cell Signaling Kinase Antibody demonstrates prominent membranous and cytoplasmic staining within lymphocyte-rich splenic compartments. The staining pattern is consistent with expression of LCK, a SRC family tyrosine kinase that functions as a critical initiator of T-cell receptor signaling and adaptive immune activation. LCK regulates phosphorylation-dependent signaling pathways that control lymphocyte activation, proliferation, differentiation, and immune communication. The strong staining observed in splenic immune cell populations supports the established role of LCK in T-cell biology, immune surveillance, and adaptive immune responses.

LCK Antibody U-2 OS Cell IF/ICC. Immunofluorescent staining of FFPE human U-2 OS cells using LCK Antibody / T-Cell Signaling Kinase Antibody demonstrates cytoplasmic and membrane-associated red fluorescence, consistent with expression of LCK. LCK is a SRC family tyrosine kinase that functions as a critical initiator of phosphorylation-dependent signaling pathways involved in lymphocyte activation, cellular communication, and immune signal transduction. The observed staining pattern is consistent with the intracellular localization expected for a non-receptor tyrosine kinase and supports the utility of this antibody for detection of LCK in immunofluorescence applications. Nuclei are counterstained with DAPI (blue). HIER: boil tissue sections in pH 6, 10 mM citrate buffer for 20 minutes and allow to cool before testing.

LCK Antibody HepG2 Cell FACS. Flow cytometric analysis of human HepG2 cells using LCK antibody demonstrates a clear rightward shift of the LCK-positive population (blue) relative to the isotype control (green) and unstained cells (red), consistent with expression of LCK. LCK is a SRC family tyrosine kinase that functions as a critical initiator of T-cell receptor signaling and phosphorylation-dependent signal transduction pathways. As a key regulator of immune cell activation and cellular communication, LCK participates in signaling networks that influence lymphocyte function, cellular responses to external stimuli, and downstream transcriptional activation. The observed fluorescence shift supports the utility of this antibody for flow cytometric detection of LCK. Red=cells alone; Green=isotype control; Blue=LCK antibody.