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Home >> Antibodies >> LAMP-3 Antibody / CD63

LAMP-3 Antibody / CD63 [clone LAMP3/529] (V3028)

  Catalog No Formulation Size Price (USD)  
Image V3028-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V3028-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V3028SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
SDS-PAGE Analysis of Purified, BSA-Free LAMP-3 Antibody (clone LAMP3/529). Confirmation of Integrity and Purity of the Antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name LAMP3/529
Purity Protein G affinity chromatography
UniProt P08962
Localization Cytoplasmic
Applications ELISA (order BSA/sodium azide-free format for coating) :
Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 0.5-1ug/ml
Limitations This LAMP-3 antibody is available for research use only.
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Description

This mAb recognizes protein of 26kDa-60kDa, which is identified as CD63/LAMP-3. Its epitope is different from that of mAb LAMP3/803 or LAMP3/968 or NKI/C3 or MX-49.129.5. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63/LAMP-3 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. It is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63/LAMP-3 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. Deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Application Notes

Optimal dilution of the LAMP-3 antibody should be determined by the researcher.

Immunogen

Recombinant human protein was used as the immunogen for the LAMP-3 antibody.

Storage

Store the LAMP-3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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