LAMC1 Antibody / Laminin gamma 1 [clone LAMC1/3162] (V8865)

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Laminin gamma 1 antibody (clone LAMC1/3162). These results demonstrate the foremost specificity of the LAMC1/3162 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Immunohistochemistry analysis of Laminin gamma 1 / LAMC1 antibody (clone LAMC1/3162) in human kidney tissue. Formalin-fixed, paraffin-embedded human kidney tissue was stained using Laminin gamma 1 antibody (clone LAMC1/3162). Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 20 minutes, followed by cooling at room temperature prior to antibody incubation. Brown chromogenic signal outlines basement membrane structures of renal tubules and glomeruli, producing a continuous linear staining pattern along tubular epithelial and glomerular capillary basement membranes, while tubular epithelial cytoplasm and interstitial stromal regions show minimal staining. This staining pattern reflects basement membrane-associated expression of Laminin gamma 1 in kidney tissue.