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Home >> Antibodies >> Lamin B1 Antibody

Lamin B1 Antibody (R30227)

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  Catalog No Formulation Size Price (USD)  
Image R30227 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human SiHa cells with Lamin B1 antibody (red) and Beta Tubulin antibody (green). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human SiHa cells with Lamin B1 antibody (red) and Beta Tubulin antibody (green). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human SiHa cells with Lamin B1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human breast cancer tissue with Lamin B1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of frozen rat intestinal tissue with Lamin B1 antibody.
Immunocytochemical staining of FFPE human A549 cells with Lamin B1 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
Western blot testing of 1) human Daudi, 2) human ThP-1, 3) human HL-60, 4) rat lung and 5) mouse lung tissue lysate with Lamin B1 antibody. Predicted molecular weight ~66 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P20700
Localization Nuclear membrane, nuclear
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
IHC (Frozen) : 0.5-1ug/ml
Immunocytochemistry : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Limitations This Lamin B1 antibody is available for research use only.
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Description

Lamin B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. They are though to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Lamin B1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 570-586 (FHQQGTPRASNRSCAIM-human) were used as the immunogen for this Lamin B1 antibody.

Storage

After reconstitution, the Lamin B1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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