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Home >> Antibodies >> Lamin B1 Antibody / Nuclear Envelope Marker Antibody

Lamin B1 Antibody / Nuclear Envelope Marker Antibody (R30227)

  Catalog No Formulation Size Price (USD)  
Image R30227 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
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Lamin B1 Antibody Human Lung Cancer IHC. Immunohistochemistry staining of FFPE human lung cancer tissue with Lamin B1 Antibody. HIER was performed using pH 8 EDTA buffer for 20 minutes prior to staining. The tissue section was incubated with Lamin B1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen detection. Lamin B1 Antibody / Nuclear Envelope Marker Antibody demonstrates nuclear staining consistent with LMNB1 expression and supports studies of nuclear envelope structure, nuclear lamina organization and chromatin-associated cellular regulation pathways.
Lamin B1 Antibody Human Breast Cancer IHC. Immunohistochemistry staining of FFPE human breast cancer tissue with Lamin B1 Antibody. HIER was performed using pH 8 EDTA buffer for 20 minutes prior to staining. The tissue section was incubated with Lamin B1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen detection. Lamin B1 Antibody / Nuclear Envelope Marker Antibody demonstrates nuclear staining consistent with LMNB1 expression and supports studies of nuclear envelope structure, chromatin organization and nuclear lamina-associated cellular regulation pathways.
Lamin B1 Antibody Human Colon Cancer IHC. Immunohistochemistry staining of FFPE human colon cancer tissue with Lamin B1 Antibody. HIER was performed using pH 8 EDTA buffer for 20 minutes prior to staining. The tissue section was incubated with Lamin B1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen detection. Lamin B1 Antibody / Nuclear Envelope Marker Antibody demonstrates nuclear staining consistent with LMNB1 expression and supports studies of nuclear envelope integrity, nuclear lamina organization and chromatin-associated gene regulation pathways.
Lamin B1 Antibody Mouse Brain IHC. Immunohistochemistry staining of FFPE mouse brain tissue with Lamin B1 Antibody. HIER was performed using pH 8 EDTA buffer for 20 minutes prior to staining. The tissue section was incubated with Lamin B1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen detection. Lamin B1 Antibody / Nuclear Envelope Marker Antibody demonstrates nuclear staining consistent with LMNB1 expression and supports studies of nuclear envelope structure, neuronal cell biology and nuclear lamina-associated regulation pathways.
Lamin B1 Antibody Rat Brain IHC. Immunohistochemistry staining of FFPE rat brain tissue with Lamin B1 Antibody. HIER was performed using pH 8 EDTA buffer for 20 minutes prior to staining. The tissue section was incubated with Lamin B1 antibody, followed by HRP-conjugated secondary antibody and DAB chromogen detection. Lamin B1 Antibody / Nuclear Envelope Marker Antibody demonstrates nuclear staining consistent with LMNB1 expression and supports studies of nuclear envelope structure, neuronal cell organization and nuclear lamina-associated chromatin regulation pathways.
Lamin B1 Antibody Human, Rat and Mouse WB. Western blot testing of human Daudi, THP-1 and HL-60 cell lysates, along with rat lung and mouse lung tissue lysates, with Lamin B1 Antibody. Lane 1: human Daudi; Lane 2: human THP-1; Lane 3: human HL-60; Lane 4: rat lung; Lane 5: mouse lung. A specific band was detected at approximately 66 kDa, consistent with the predicted molecular weight of LMNB1. Lamin B1 Antibody / Nuclear Envelope Marker Antibody detects endogenous Lamin B1 expression and supports studies of nuclear envelope structure, nuclear lamina organization and chromatin-associated cellular regulation pathways.
amin B1 Antibody Human, Rat and Mouse Cell WB. Western blot testing of human HeLa, K562 and SH-SY5Y cell lysates, rat spleen tissue lysate, rat PC-12 cell lysate and mouse RAW264.7 cell lysate with Lamin B1 Antibody. Lane 1: human HeLa; Lane 2: human K562; Lane 3: human SH-SY5Y; Lane 4: rat spleen; Lane 5: rat PC-12; Lane 6: mouse RAW264.7. A specific band was detected at approximately 66 kDa, consistent with the predicted molecular weight of LMNB1. Lamin B1 Antibody / Nuclear Envelope Marker Antibody detects endogenous Lamin B1 expression and supports studies of nuclear envelope organization, nuclear lamina structure and chromatin-associated cellular regulation pathways.
Lamin B1 Antibody U-2 OS Cell IF. Immunofluorescent staining of FFPE human U-2 OS cells with Lamin B1 Antibody (green). HIER was performed by steaming tissue sections in pH 6 citrate buffer for 20 minutes prior to staining. Nuclei were counterstained with DAPI (blue). Lamin B1 Antibody / Nuclear Envelope Marker Antibody demonstrates nuclear envelope staining consistent with LMNB1 localization and supports studies of nuclear lamina organization, nuclear structure regulation and chromatin-associated cellular pathways.
Lamin B1 Antibody Human Lung Cancer IF. Immunofluorescent staining of FFPE human lung cancer tissue with Lamin B1 Antibody (red). HIER was performed by steaming tissue sections in pH 8 EDTA buffer for 20 minutes prior to staining. Nuclei were counterstained with DAPI (blue). Lamin B1 Antibody / Nuclear Envelope Marker Antibody demonstrates nuclear staining consistent with LMNB1 expression and supports studies of nuclear lamina organization, nuclear envelope structure and chromatin-associated cellular regulation pathways.
Lamin B1 Antibody HeLa Cell IP. Immunoprecipitation of Lamin B1 protein from 500 ug of human HeLa whole cell lysate using 2 ug of Lamin B1 Antibody. Immunoprecipitated protein was analyzed by Western blot, with input lysate and control IgG samples included for comparison. Lamin B1 was successfully enriched and detected at approximately 66 kDa, consistent with the predicted molecular weight of LMNB1. Lamin B1 Antibody / Nuclear Envelope Marker Antibody supports analysis of nuclear lamina protein interactions, nuclear envelope organization and chromatin-associated cellular regulation pathways.
Lamin B1 Antibody Mouse Brain IF. Immunofluorescent staining of FFPE mouse brain tissue with Lamin B1 Antibody at 5 ug/ml. HIER was performed using pH 8 EDTA buffer, followed by blocking with 10% goat serum. The tissue section was incubated with rabbit anti-Lamin B1 antibody overnight at 4°C, followed by Cy3 conjugated goat anti-rabbit IgG secondary antibody (red). Nuclei were counterstained with DAPI (blue). Lamin B1 Antibody / Nuclear Envelope Marker Antibody demonstrates nuclear envelope staining consistent with LMNB1 localization and supports studies of nuclear lamina organization, nuclear structure and chromatin-associated cellular regulation pathways.
Lamin B1 Antibody Rat Brain IF. Immunofluorescent staining of FFPE rat brain tissue with Lamin B1 Antibody at 5 ug/ml. HIER was performed using pH 8 EDTA buffer, followed by blocking with 10% goat serum. The tissue section was incubated with rabbit anti-Lamin B1 antibody overnight at 4°C, followed by Cy3 conjugated goat anti-rabbit IgG secondary antibody (red). Nuclei were counterstained with DAPI (blue). Lamin B1 Antibody / Nuclear Envelope Marker Antibody demonstrates nuclear envelope staining consistent with LMNB1 localization and supports studies of nuclear lamina organization, nuclear architecture and chromatin-associated cellular regulation pathways.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P20700
Localization Nucleus lamina
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Immunoprecipitation : 2ug per 500ug of lysate
Limitations This Lamin B1 Antibody / Nuclear Envelope Marker Antibody is available for research use only.
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Description

Lamin B1 Antibody / Nuclear Envelope Marker Antibody detects LMNB1, a major structural component of the nuclear lamina located beneath the inner nuclear membrane. Lamin B1 belongs to the type V intermediate filament protein family and forms a filamentous network that provides mechanical support to the nucleus. This nuclear scaffold helps regulate nuclear shape, chromatin organization, DNA replication and gene expression. Because of its stable localization at the nuclear periphery, Lamin B1 is widely studied as a marker of nuclear envelope structure and nuclear organization.

Lamin B1 plays an important role in maintaining nuclear architecture and coordinating interactions between chromatin and the nuclear envelope. The nuclear lamina influences genome organization by anchoring specific chromosomal regions and regulating access to transcriptional machinery. Changes in Lamin B1 expression and distribution can affect nuclear stability, cellular function and responses to stress. Lamin B1 Antibody is commonly used by researchers studying nuclear morphology, nuclear membrane biology, chromatin organization and cellular structural regulation.

Research involving LMNB1 spans multiple areas including cell biology, aging, cancer biology and neurological research. Reduced Lamin B1 expression is frequently studied as a feature associated with cellular senescence, while altered nuclear lamina organization has been connected with changes in gene regulation and disease-associated cellular states. LMNB1 duplication and abnormal Lamin B1 regulation are also investigated in neurological disorders, highlighting the importance of nuclear envelope integrity in specialized cell types.

Lamin B1 is frequently analyzed alongside other nuclear markers to evaluate nuclear organization, cell identity and structural changes during biological processes. Studies of nuclear lamina proteins provide insight into how nuclear architecture influences genome function, cell division and cellular aging pathways. As a core nuclear envelope protein, Lamin B1 remains an important target for understanding relationships between nuclear structure and cellular regulation.

Lamin B1 Antibody applications include detection of LMNB1 expression, localization and regulation in biological samples using techniques such as Western blot, immunohistochemistry, immunofluorescence and related protein analysis methods. NSJ Bioreagents provides Lamin B1 Antibody / Nuclear Envelope Marker Antibody for researchers studying nuclear envelope biology, chromatin organization, cellular senescence and nuclear structure regulation.

Lamin B1 Antibody detects a nuclear lamina protein involved in nuclear envelope structure, chromatin organization and cellular regulation, making it a useful marker for research areas featured in our Cell Biology Antibodies page.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Lamin B1 Antibody / Nuclear Envelope Marker Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 570-586 (FHQQGTPRASNRSCAIM-human) were used as the immunogen for this Lamin B1 antibody.

Storage

After reconstitution, the Lamin B1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

LMNB1 Antibody, Lamin-B1 Antibody, Nuclear Lamin B1 Antibody, Nuclear Lamina Protein Antibody, Nuclear Envelope Protein Antibody, Senescence Marker Antibody

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