Lambda Light Chain Antibody LcN-2 | V2153 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	V2153-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	519
Formulation
	V2153-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	229
Formulation
	V2153SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	519
Formulation
	V2153IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	519
Formulation

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IHC testing of tonsil stained with Lambda Light Chain antibody (LcN-2). Note cell membrane & cytoplasmic staining.

Western blot testing of human samples using Lambda Light Chain antibody (LcN-2).

Confocal immunofluorescent testing of Ramos cells with Alexa Fluor 488 conjugated Lambda Light Chain antibody (green) and DAPI nuclear counterstain (blue).

Species Reactivity	Human
Format	Purified
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG2a, kappa
Clone Name	LcN-2
Purity	Protein G affinity chromatography
Buffer	1X PBS, pH 7.4
Gene ID	3535
Localization	Cell surface, cytoplasmic and secreted
Applications	Flow cytometry : 0.5-1ug/10^6 cells Immunofluorescence : 1-2ug/ml Western blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT
Limitations	This Lambda Light Chain antibody is available for research use only.
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Description

This antibody is specific to lambda light chain of immunoglobulin and shows no cross-reaction with kappa light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.