Lambda Light Chain Antibody LcN-2 / Mouse Monoclonal [clone LcN-2] (V2153)

Description

Lambda Light Chain Antibody clone LcN-2 is a mouse monoclonal reagent for detecting human immunoglobulins containing lambda light chains. Immunoglobulin molecules consist of two identical heavy chains and two identical light chains, with each antibody containing either kappa or lambda light chains. Lambda light chains contribute to the antigen-binding region of immunoglobulins and are expressed by a subset of mature B cells and antibody-secreting plasma cells.

Human lambda light chains are encoded by gene segments within the immunoglobulin lambda locus on chromosome 22. Rearrangement of variable and joining gene segments contributes to antibody diversity, while the resulting lambda chains associate with immunoglobulin heavy chains to form functional antibodies. Lambda light chains can occur as components of membrane-bound B-cell receptors, secreted immunoglobulins, or free light chains.

Kappa and lambda light-chain expression is commonly examined in studies of B-cell populations and immunoglobulin production. Individual mature B cells generally express immunoglobulins containing one light-chain type, while polyclonal populations contain cells expressing either kappa or lambda chains. Restricted light-chain expression can therefore provide useful information in research examining B-cell clonality and plasma-cell populations.

Lambda light-chain detection is relevant to immunology, B-cell biology, antibody research, and studies of lymphoid and plasma-cell disorders. Free monoclonal immunoglobulin light chains have historically been referred to as Bence Jones proteins and are studied in connection with abnormal immunoglobulin production. Comparing lambda and kappa expression can further support investigation of immunoglobulin light-chain distribution and clonal lymphoid populations.

A Lambda Light Chain Antibody such as clone LcN-2 provides a mouse monoclonal reagent generated using purified human immunoglobulin. The antibody is specific for human lambda light chain and shows no cross-reaction with kappa light chain or any of the five immunoglobulin heavy-chain classes, supporting selective detection of lambda-expressing immunoglobulins and cells. NSJ Bioreagents offers LcN-2 for research examining immunoglobulin expression, light-chain restriction, B-cell biology, and related immune processes.

For a recombinant mouse monoclonal targeting the same immunoglobulin light-chain class, see our Lambda Light Chain Antibody rLLC/1738 / IGL Antibody page.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Lambda Light Chain Antibody LcN-2 to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Purified human Ig was used as the immunogen for this Lambda Light Chain Antibody LcN-2.

Storage

Store the Lambda Light Chain Antibody LcN-2 at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

IGL antibody, Immunoglobulin lambda light chain antibody, Lambda light chain antibody, Lambda chain antibody, Ig lambda antibody, Immunoglobulin light chain lambda antibody