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Home >> Antibodies >> Lambda Light Chain Antibody

Lambda Light Chain Antibody [clone HP6054] (V3151CF488)

  Catalog No Formulation Size Price (USD)  
Image V3151CF488-100T 500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 Tests 519
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(Left) Immunofluorescent staining of permeabilized human Ramos cells with CF488-labeled Lambda Light Chain antibody (clone HP6054, green) and DAPI (blue). (Right) Negative control.
Availability 1-3 business days
Species Reactivity Human
Format CF488 Conjugate
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name HP6054
Purity Protein G affinity chromatography
UniProt P01701 , P01842
Localization Cell surface, cytoplasmic, secreted
Applications Flow cytometry : 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood
Immunofluorescence : 1-2ug/ml
Limitations This Lambda Light Chain antibody is available for research use only.
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Description

This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Application Notes

Optimal dilution of the Lambda Light Chain antibody should be determined by the researcher.

Immunogen

Purified human IgG myeloma proteins were used as the immunogen for this Lambda Light Chain antibody.

Storage

Store the Lambda Light Chain antibody at 2-8oC, protected from light.

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