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Home >> Antibodies >> Lactoferrin Antibody / LTF

Lactoferrin Antibody / LTF (RQ4253)

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  Catalog No Formulation Size Price (USD)  
Image RQ4253 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) human placenta, 2) rat spleen and 3) mouse spleen tissue lysate with Lactoferrin antibody at 0.5ug/ml. Predicted molecular weight: ~78 kDa (isoform 1) and ~73 kDa (isoforms 2).
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Western blot testing of 1) human placenta, 2) rat spleen and 3) mouse spleen tissue lysate with Lactoferrin antibody at 0.5ug/ml. Predicted molecular weight: ~78 kDa (isoform 1) and ~73 kDa (isoforms 2).
IHC testing of FFPE human intestinal cancer tissue with Lactoferrin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human lung cancer tissue with Lactoferrin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse spleen tissue with Lactoferrin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human prostate cancer tissue with Lactoferrin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human SiHa cells with Lactoferrin antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Lactoferrin antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P02788
Localization Secreted, Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Direct ELISA : 0.1-0.5ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/10^6 cells
Limitations This Lactoferrin antibody is available for research use only.
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Description

Lactoferrin (LF), also known as lactotransferrin (LTF), is a multifunctional protein of the transferrin family. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides.

Application Notes

Optimal dilution of the Lactoferrin antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids D529-K710 was used as the immunogen for the Lactoferrin antibody.

Storage

After reconstitution, the Lactoferrin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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