L-Plastin Antibody / Leukocyte Cytoskeletal Marker Antibody (RQ4893)

L-Plastin Antibody Human U-2 OS IF. Immunofluorescent staining of FFPE human U-2 OS cells with L-Plastin antibody revealed predominantly cytoplasmic staining with punctate and peripheral localization consistent with the distribution of L-plastin (LCP1), a calcium-regulated actin-bundling protein involved in cytoskeletal remodeling and cell migration. L-plastin is best known as a leukocyte marker but is also expressed in several malignancies, where it contributes to invasive and metastatic behavior. Green fluorescence indicates L-Plastin expression, while nuclei are counterstained with DAPI (blue). Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to immunostaining.

L-Plastin Antibody Human Lymphocytic Tumor IHC. IHC staining of FFPE human lymphocytic tumor tissue with L-Plastin antibody demonstrated widespread cytoplasmic and membranous staining of neoplastic lymphoid cells, consistent with the expression pattern of L-plastin (LCP1), a leukocyte-specific actin-bundling protein involved in immune cell activation and migration. L-plastin serves as a marker of hematopoietic cells and is frequently expressed in lymphoid malignancies. The diffuse staining pattern observed is consistent with the cytoskeletal remodeling and migratory properties characteristic of lymphocytic tumors. Immunostaining was performed with L-Plastin antibody at 1 ug/ml following heat-induced epitope retrieval by boiling tissue sections in pH 6, 10 mM citrate buffer for 10-20 minutes and allowing them to cool before testing.

L-Plastin Antibody Human Tonsil IHC. IHC staining of FFPE human tonsil with L-Plastin antibody demonstrated strong cytoplasmic and membranous staining of lymphoid cells within the tonsillar parenchyma, consistent with the expression pattern of L-plastin (LCP1), a leukocyte-specific actin-bundling protein involved in immune cell activation and migration. L-plastin is broadly expressed in hematopoietic cells and serves as a useful marker of lymphocytes and other leukocyte populations. The widespread staining observed in tonsillar immune cells is consistent with the active cytoskeletal remodeling required for antigen recognition and immune surveillance. Immunostaining was performed with L-Plastin antibody at 1 ug/ml following heat-induced epitope retrieval by boiling tissue sections in pH 6, 10 mM citrate buffer for 10-20 minutes and allowing them to cool before testing.

L-Plastin Antibody Human Tonsillar Follicles IHC. IHC staining of FFPE human tonsil with L-Plastin antibody demonstrated strong cytoplasmic and membranous staining of lymphoid cells within tonsillar follicles and interfollicular regions, consistent with the expression pattern of L-plastin (LCP1), a leukocyte-specific actin-bundling protein involved in immune cell activation and migration. L-plastin is broadly expressed in lymphocytes and other hematopoietic cells, making it a useful marker of immune cell populations. The prominent staining observed throughout the lymphoid follicles is consistent with the active cytoskeletal remodeling required for antigen recognition, cell trafficking, and immune surveillance. Immunostaining was performed with L-Plastin antibody at 1 ug/ml following heat-induced epitope retrieval by boiling tissue sections in pH 6, 10 mM citrate buffer for 10-20 minutes and allowing them to cool before testing.

L-Plastin Antibody Human and Rodent Immune Cell WB. Western blot analysis of LCP1 expression was performed using anti-L-Plastin antibody in lysates from human CCRF-CEM, HEK293, HL-60, and THP-1 cells, rat spleen, mouse spleen, and mouse RAW264 cells. L-plastin, also known as Lymphocyte Cytosolic Protein 1 (LCP1), is a calcium-regulated actin-bundling protein predominantly expressed in hematopoietic cells and involved in leukocyte migration, immune cell activation, and cytoskeletal remodeling. Lane 1: CCRF-CEM. Lane 2: HEK293. Lane 3: HL-60. Lane 4: THP-1. Lane 5: rat spleen. Lane 6: mouse spleen. Lane 7: mouse RAW264 lysate. A prominent immunoreactive band is detected at approximately 70 kDa in all samples, corresponding to the predicted molecular weight of L-plastin. Strong expression in immune cell lines and lymphoid tissues is consistent with the established role of L-plastin as a marker of hematopoietic cells and mediator of leukocyte function. These results support the utility of L-Plastin Antibody for studies of innate immunity, inflammation, hematopoiesis, and cell migration.

L-Plastin Antibody Human U-2 OS FACS. Flow cytometric analysis of LCP1 expression was performed using L-Plastin antibody in human U-2 OS cells. L-plastin, also known as Lymphocyte Cytosolic Protein 1 (LCP1), is a calcium-regulated actin-bundling protein involved in cytoskeletal remodeling, cell adhesion, and migration. Cells were blocked with goat sera and stained with L-Plastin antibody at 1 ug/million cells. Red = cells alone, Green = isotype control, Blue = L-Plastin antibody. A marked rightward shift of the L-Plastin antibody histogram relative to the isotype control demonstrates specific detection of LCP1 expression in U-2 OS cells. These results support the utility of L-Plastin Antibody for studies of cytoskeletal dynamics, immune cell markers, and cancer cell migration.