Kv1.2 Antibody / Voltage-Gated Potassium Channel Antibody (R31982)

Kv1.2 Antibody Human U-2 OS Cell IF. Immunofluorescent staining of FFPE human U-2 OS cells with Kv1.2 antibody (green). Heat-induced epitope retrieval (HIER) was performed by steaming sections in pH 6 citrate buffer for 20 minutes prior to staining. Nuclei are counterstained with DAPI (blue). Kv1.2 immunoreactivity is observed predominantly along the plasma membrane and within the cytoplasm, consistent with the expected localization of this voltage-gated potassium channel involved in regulating neuronal membrane excitability and action potential repolarization.

Kv1.2 Antibody A431 Cell FACS. Flow cytometry testing of human A431 cells with Kv1.2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Kv1.2 antibody.

Kv1.2 Antibody Rat C6 Cell FACS. Flow cytometry testing of rat C6 cells with Kv1.2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Kv1.2 antibody.

Kv1.2 Antibody Multi-Species WB. Western blot testing of 1) rat kidney, 2) rat brain, 3) mouse brain and 4) mouse kidney lysates with Kv1.2 antibody. A single band is detected at approximately 57 kDa in all four samples, consistent with the predicted molecular weight of Kv1.2. The results demonstrate specific detection of endogenous Kv1.2 across both rat and mouse tissues, with comparable expression observed in brain and kidney lysates.

Kv1.2 Antibody Human Cerebral Cortex and Hippocampus IHC. Immunohistochemical staining of FFPE human cerebral cortex and hippocampus tissue with Kv1.2 antibody at 2 ug/ml. Heat-induced epitope retrieval (HIER) was performed by boiling tissue sections in pH 8 EDTA buffer prior to staining. Kv1.2 immunoreactivity is observed predominantly within neurons and the surrounding neuropil, consistent with the expected localization of this voltage-gated potassium channel involved in regulating neuronal membrane excitability and action potential repolarization. Cell nuclei are counterstained with hematoxylin (blue).