Ku80 Antibody / Cell Cycle Regulation Antibody (R31953)

Ku80 Antibody / Cell Cycle Regulation Antibody for WB. Western blot analysis of Ku80 (XRCC5) in multiple cell and tissue lysates. Lane 1: rat testis lysate, Lane 2: rat thymus lysate, Lane 3: human HeLa cell lysate, Lane 4: mouse NIH3T3 cell lysate. A band is detected at approximately 80-86 kDa, consistent with the predicted molecular weight of Ku80 (XRCC5). The consistent expression across proliferative tissues and cell lines supports the role of Ku80 in cell cycle regulation, where coordination of DNA repair is required for proper progression through cell division.

Ku80 Antibody / Cell Cycle Regulation Antibody. Immunohistochemistry analysis of human lung cancer tissue using Ku80 antibody. Formalin-fixed, paraffin-embedded sections show HRP-DAB brown nuclear staining in tumor epithelial cells, consistent with expression of Ku80 (XRCC5) in proliferative cell populations. The staining is enriched within densely cellular tumor regions, reflecting the role of Ku80 in coordinating DNA repair with cell cycle progression. Hematoxylin counterstain highlights nuclei (blue), providing contrast to the Ku80-positive nuclear signal and supporting its function in actively dividing cancer cells.

IHC testing of frozen human placenta with Ku80 antibody.

Ku80 Antibody / Cell Cycle Regulation Antibody. Immunofluorescence analysis of formalin-fixed, paraffin-embedded human U-2 OS cells using Ku80 antibody. The antibody signal (red) shows predominant nuclear localization consistent with Ku80 (XRCC5) expression in actively cycling cells. DAPI (blue) marks nuclei, demonstrating strong overlap of Ku80 signal with nuclear regions. The staining pattern supports the role of Ku80 in coordinating DNA repair with cell cycle progression, particularly in proliferative cell populations.

Flow cytometry testing of human SiHa cells with Ku80 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ku80 antibody.

Ku80 Antibody / Cell Cycle Regulation Antibody for WB. Western blot analysis of Ku80 (XRCC5) in multiple cell and tissue lysates. Lane 1: human HeLa cell lysate, Lane 2: human A549 cell lysate, Lane 3: human 293T cell lysate, Lane 4: human HepG2 cell lysate, Lane 5: rat brain tissue lysate, Lane 6: mouse brain tissue lysate. A band is detected at approximately 80-86 kDa, consistent with the predicted molecular weight of Ku80 (XRCC5). The strong and consistent expression across proliferative cell lines and differentiated tissues supports the role of Ku80 in coordinating DNA repair with cell cycle progression, where maintenance of genomic integrity is required across diverse cellular contexts.