Ku80 Antibody / DNA Damage Response Signaling Antibody (R30724)

Ku80 Antibody / DNA Damage Response Signaling Antibody. Immunohistochemistry analysis of human breast cancer tissue using Ku80 antibody. Formalin-fixed, paraffin-embedded sections show strong HRP-DAB brown nuclear staining in tumor epithelial cells, consistent with activation of DNA damage response pathways involving Ku80 (XRCC5). The staining is prominent within densely cellular tumor regions, reflecting engagement of DNA repair and stress signaling mechanisms in proliferating cancer cells. Hematoxylin counterstain highlights nuclei (blue), providing contrast to the Ku80-positive nuclear signal.

IHC staining of FFPE human breast cancer with Ku80 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast cancer with Ku80 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lung cancer with Ku80 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human intestinal cancer with Ku80 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

ICC testing of Ku80 antibody and HeLa cells.

Immunofluorescent staining of FFPE human A549 cells with Ku80 antibody. HIER: steam section in pH6 citrate buffer for 20 min.

Ku80 Antibody / DNA Damage Response Signaling Antibody. Immunofluorescence analysis of formalin-fixed, paraffin-embedded human intestinal cancer tissue using Ku80 antibody. The antibody signal (red) shows prominent nuclear staining within tumor epithelial cells, consistent with activation of DNA damage response pathways involving Ku80 (XRCC5). Signal intensity is enriched in densely cellular tumor regions, reflecting engagement of DNA repair and stress signaling mechanisms. The staining pattern supports the role of Ku80 as a nuclear mediator of DNA damage response in cancer tissue.

Ku80 Antibody / DNA Damage Response Signaling Antibody for WB. Western blot analysis of human cell lysates using Ku80 antibody. Lane 1: jurkat cell lysate, Lane 2: CEM cell lysate, Lane 3: raji cell lysate, Lane 4: COLO320 cell lysate, Lane 5: HT1080 cell lysate. A band is detected at approximately 80-86 kDa, consistent with the predicted molecular weight of Ku80 (XRCC5). The consistent band intensity across multiple hematopoietic and cancer-derived cell lines reflects the widespread expression of Ku80 and aligns with its role as a nuclear DNA damage response protein involved in double-strand break repair signaling.

Flow cytometry testing of human SiHa cells with Ku80 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ku80 antibody.