Ku80 Antibody for IHC | XRCC5 Immunohistochemistry Ab |V6131

	Catalog No	Formulation	Size	Price (USD)
	V6131-100UG	Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide	100 ug	719.95
Formulation
	V6131-20UG	Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide	20 ug	389.4
Formulation

Ku80 Antibody for IHC / XRCC5 Immunohistochemistry Antibody. Immunohistochemistry analysis of human tissue microarray (TMA) using Ku80 antibody clone MSVA-880M demonstrates widespread nuclear HRP-DAB brown staining across multiple normal and cancer tissues. Strong nuclear staining is observed in epithelial, stromal, and lymphoid cell populations, with cell-type-dependent intensity and enhanced signal in proliferative and tumor tissues. The staining pattern highlights consistent XRCC5 (Ku80) localization across diverse tissue types, with clear contrast between normal and malignant samples. These results align with expression data reported in the Human Protein Atlas and support the use of this antibody for tissue-based detection of nuclear DNA repair protein expression.

Species Reactivity	Human
Format	Purified
Host	Mouse
Clonality	Recombinant Mouse Monoclonal
Isotype	Mouse IgG1, kappa
Clone Name	MSVA-880M
UniProt	P13010
Localization	Nucleoplasm, Nucleus
Applications	Immunohistochemistry (FFPE) : 1:100-1:200
Limitations	This XRCC5/Ku80 antibody is available for research use only.
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Description

Ku80 (XRCC5) is a nuclear protein widely expressed across human tissues and is readily detectable in formalin-fixed, paraffin-embedded tissue sections, making it highly suitable for immunohistochemistry-based analysis. Ku80 Antibody for IHC is specifically designed for immunohistochemistry applications, enabling clear visualization of XRCC5 expression and nuclear staining patterns in tissue sections. This antibody supports evaluation of protein distribution across normal and diseased tissues, allowing direct comparison of staining within intact tissue architecture.

Ku80 Antibody for IHC is uniquely positioned for tissue-based studies where preservation of morphology and cellular context is essential. XRCC5 antibody, also known as Ku80 antibody, produces predominantly nuclear HRP-DAB brown staining in epithelial, stromal, and lymphoid cell populations, consistent with its intracellular localization. In immunohistochemistry analysis, staining is typically observed as distinct nuclear signal, allowing clear identification of positive cells within complex tissue environments and across diverse histological structures.

In tumor tissues, Ku80 Antibody for IHC frequently shows strong nuclear staining in malignant cell populations, often with higher intensity compared to surrounding non-neoplastic tissue. This contrast supports its use in studies comparing tumor versus normal expression patterns and provides insight into differences in cellular activity within the tumor microenvironment. In normal tissues, staining is generally more uniform and consistent across cell types, reflecting baseline XRCC5 expression and allowing reliable interpretation of tissue-specific distribution.

In human tissue microarray (TMA) analysis, Ku80 Antibody for IHC demonstrates consistent nuclear staining across a broad panel of normal and cancer tissues, including epithelial, lymphoid, and glandular compartments. The widespread yet cell-type-dependent staining intensity highlights the utility of XRCC5 detection for comparative tissue analysis, where differences between normal and malignant tissues can be readily observed. This reproducible staining across diverse tissue cores supports reliable evaluation of XRCC5 expression in large-scale tissue screening studies.

The recombinant mouse monoclonal format supports consistent staining performance and low background in FFPE specimens, enabling clear signal detection and reproducible results across experiments. Ku80 Antibody for IHC provides strong nuclear staining with minimal non-specific signal, allowing accurate differentiation between positive and negative cells in complex tissue environments. This level of clarity is particularly important for histological evaluation and interpretation of staining patterns in research settings.

Ku80 Antibody for IHC provides a focused tool for tissue-level analysis of XRCC5 expression, enabling detailed examination of nuclear localization within intact tissue architecture. Its consistent nuclear staining, reproducibility across tissue types, and ability to highlight differences between normal and cancer tissues make it well suited for immunohistochemistry studies requiring clear, interpretable, and biologically meaningful staining patterns.

Application Notes

1. Optimal dilution of the Ku80 Antibody for IHC / XRCC5 Immunohistochemistry Antibody should be determined by the researcher.

2. This XRCC5/Ku80 antibody is recombinantly produced by expression in CHO cells.

3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Immunogen

A recombinant fragment (around amino acids 300-500) of human XRCC5 (exact sequence is proprietary) was used as the immunogen for the Ku80 Antibody for IHC / XRCC5 Immunohistochemistry Antibody.