- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Ku70 + Ku80 Antibody / XRCC6 + XRCC5 Nuclear DNA Repair Antibody [clone LKAP1-1] (V7202)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V7202-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 559 | |
|
|
V7202-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 259 | |
|
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V7202SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Species Reactivity | Human |
| Format | Purified |
| Host | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | LKAP1-1 |
| Purity | Protein G purified |
| Buffer | 1X PBS, pH 7.4 |
| Gene ID | 2547 |
| Localization | Nuclear |
| Applications | Flow Cytometry : 0.5-1ug/10^6 cells Immunofluorescence : 1-2ug/ml |
| Limitations | This Ku70 + Ku80 antibody is available for research use only. |
|
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Ku70 (XRCC6) and Ku80 (XRCC5) form a highly conserved nuclear heterodimer that functions as a central component of the non-homologous end joining pathway responsible for repairing DNA double-strand breaks. Ku70 + Ku80 Antibody (clone LKAP1-1) recognizes this DNA repair complex and is particularly well suited for visualizing nuclear protein localization and chromatin-associated staining patterns. The Ku heterodimer is predominantly localized within the nucleus, where it binds DNA ends and recruits additional repair machinery, making it a valuable marker for imaging-based studies of genomic stability and cellular stress responses.
Ku70 + Ku80 antibody, also referred to as XRCC6 antibody or XRCC5 antibody, detects ubiquitously expressed proteins whose nuclear distribution can change in response to DNA damage and cell cycle progression. This makes the Ku complex especially useful for immunofluorescence applications where spatial localization and staining pattern are critical for interpretation. Ku70 and Ku80 typically exhibit diffuse to punctate nuclear staining consistent with chromatin association, enabling researchers to assess nuclear organization and DNA repair dynamics at the single-cell level.
The clone LKAP1-1 antibody supports intracellular detection of the Ku heterodimer and provides strong performance in workflows that require clear visualization of nuclear targets. While it can be used in intracellular flow cytometry following fixation and permeabilization, its primary strength lies in applications that emphasize cellular localization, including imaging-based analysis of DNA repair protein distribution. This makes it particularly useful for studies examining nuclear architecture, DNA damage signaling, and chromosomal integrity in both normal and transformed cells.
Ku70 and Ku80 are members of the DNA repair protein family and interact with DNA-dependent protein kinase to facilitate efficient DNA end joining. Their stable nuclear localization and essential role in maintaining genome integrity make them reliable markers for studying DNA repair mechanisms and cellular stress pathways. Ku70 + Ku80 Antibody (clone LKAP1-1) provides a robust tool for detecting XRCC6 and XRCC5 in applications that prioritize nuclear localization, imaging clarity, and biologically meaningful staining patterns.
Titering of the Ku70 + Ku80 Antibody / XRCC6 + XRCC5 Nuclear DNA Repair Antibody may be required for optimal performance.
A human cell nuclear fraction was used as the immunogen for this Ku70 + Ku80 Antibody / XRCC6 + XRCC5 Nuclear DNA Repair Antibody.
Store the Ku70 + Ku80 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
XRCC6 antibody, XRCC5 antibody, Ku70 antibody, Ku80 antibody, Ku heterodimer antibody
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