- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Ksp-Cadherin Antibody / Human Kidney-Enriched Detection Antibody [clone CDH16/8800R] (V4437)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V4437-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
|
|
V4437-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
|
|
V4437SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | CDH16/8800R |
| Purity | Protein A affinity |
| UniProt | O75309 |
| Localization | Cell surface, Cytoplasm |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT Western Blot : 2-4ug/ml |
| Limitations | This Ksp-Cadherin Antibody / Human Kidney-Enriched Detection Antibody is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
Related Products
|
Cadherin-16 (CDH16), commonly known as Ksp-Cadherin, is a kidney-restricted member of the cadherin superfamily that mediates calcium-dependent cell-cell adhesion in renal epithelial cells. Ksp-Cadherin is localized predominantly to the basolateral membrane of tubular epithelial cells, where it contributes to epithelial polarity, structural cohesion, and maintenance of nephron architecture. This Ksp-Cadherin antibody is designed to detect Cadherin-16 with strong performance in human kidney tissue, supporting a human kidney-enriched detection profile for studies focused on renal epithelial biology.
Ksp-Cadherin antibody, also referred to as CDH16 antibody and Cadherin-16 antibody in the literature, recognizes a transmembrane glycoprotein with highly selective expression within the kidney, most prominently in distal tubules and collecting duct epithelium. A defining feature of this antibody is the strong detection of Cadherin-16 in human kidney lysate by western blot, with minimal or no detectable signal in kidney lysates from mouse, rat, hamster, guinea pig, and rabbit under the tested conditions. This pattern supports a human kidney-enriched detection profile and provides a clear experimental distinction from antibodies with broader cross-species reactivity, enabling focused analysis of human renal tissue.
Structurally, Ksp-Cadherin contains multiple extracellular cadherin repeat domains that mediate calcium-dependent adhesion, a single transmembrane region, and a cytoplasmic domain involved in intracellular signaling and cytoskeletal organization. Unlike widely expressed cadherins such as E-Cadherin and N-Cadherin, CDH16 exhibits a highly restricted expression profile, reflecting its specialized function within renal epithelial compartments. The protein undergoes glycosylation, resulting in a slightly elevated apparent molecular weight on SDS-PAGE, a consistent and reproducible feature observed in kidney tissue lysates.
Functionally, CDH16 plays a critical role in maintaining epithelial integrity and polarity within kidney tubules, supporting tight intercellular junctions required for proper tubular structure and function. Its expression is closely associated with differentiated renal epithelial cells, making it a reliable marker for kidney-specific cell populations and epithelial lineage identity. The strong detection observed in human kidney lysate reinforces its relevance in studies of renal physiology and highlights its utility in identifying kidney-derived cells in complex tissue samples.
Immunohistochemical analysis of human kidney tissue further supports this expression pattern, with distinct membranous staining of renal tubular epithelial cells consistent with the known localization of Ksp-Cadherin. This tissue-level validation complements the western blot findings and provides morphological confirmation of target expression in human kidney. The combined biochemical and histological evidence strengthens confidence in the specificity and applicability of this antibody in human renal studies.
Clone CDH16/8800R is a recombinant rabbit monoclonal antibody designed to recognize Cadherin-16 with high specificity. Its strong and selective detection in human kidney tissue, combined with minimal signal in non-human samples under the tested conditions, provides a clear differentiation as a human kidney-enriched reagent. This makes it particularly valuable for renal research, studies of kidney epithelial differentiation, and investigations requiring precise detection of CDH16 in human tissue.
This antibody is part of a broader antibody panel offered by NSJ Bioreagents.
Optimal dilution of the Ksp-Cadherin Antibody / Human Kidney-Enriched Detection Antibody should be determined by the researcher.
A recombinant partial protein sequence (within amino acids 350-550) from the human protein was used as the immunogen for the Ksp-Cadherin antibody.
Aliquot the Ksp-Cadherin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
CDH16 antibody, Cadherin-16 antibody, Ksp-Cadherin kidney antibody, CDH16 renal antibody, Ksp-Cadherin WB antibody
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
