Krueppel-Like Factor 5 Antibody / BTEB2 Antibody (F54921)

Krueppel-Like Factor 5 Antibody Human Testis IHC. Immunohistochemical staining of FFPE human testis tissue using Krueppel-Like Factor 5 Antibody. Heat-induced epitope retrieval was performed in pH 6.0 citrate buffer prior to incubation with the primary antibody overnight at 4°C. An HRP-conjugated secondary antibody with DAB chromogen was used for visualization. Strong nuclear staining is observed within spermatogenic cells of the seminiferous tubules, with minimal background staining in the surrounding interstitial tissue, consistent with the expected localization of the KLF5 transcription factor in proliferating germ cells. These results demonstrate that Krueppel-Like Factor 5 Antibody, a BTEB2 Antibody, specifically detects endogenous KLF5 in FFPE human testis tissue.

Krueppel-Like Factor 5 Antibody Human U-251 Cell IF. Immunofluorescent staining of fixed and permeabilized human U-251 cells using Krueppel-Like Factor 5 Antibody (green). Actin filaments were co-stained with an anti-actin antibody (red), and nuclei were counterstained with DAPI (blue). KLF5 immunoreactivity is concentrated predominantly within the nuclei, with weaker punctate cytoplasmic staining, while the actin cytoskeleton clearly outlines cell morphology. These results demonstrate that Krueppel-Like Factor 5 Antibody, a BTEB2 Antibody, specifically detects endogenous KLF5 in human glioblastoma cells and confirms the expected nuclear localization of this transcription factor.

Krueppel-Like Factor 5 Antibody Human CCRF-CEM Cell WB. Western blot testing of human CCRF-CEM cell lysate using Krueppel-Like Factor 5 Antibody. A distinct band is detected at approximately 55 kDa, consistent with one of the reported KLF5 isoforms and the known electrophoretic migration of the protein. Although the predicted molecular weight ranges from approximately 34-51 kDa due to multiple isoforms, post-translational modifications may contribute to a higher apparent molecular weight. These results demonstrate that Krueppel-Like Factor 5 Antibody, a BTEB2 Antibody, specifically detects endogenous KLF5 in human CCRF-CEM cells.

Krueppel-Like Factor 5 Antibody Human HeLa Cell FACS. Flow cytometric analysis of fixed and permeabilized human HeLa cells using Krueppel-Like Factor 5 Antibody. Blue: isotype control. Green: Krueppel-Like Factor 5 Antibody. The clear rightward shift of the green histogram relative to the isotype control demonstrates specific intracellular detection of endogenous KLF5. These results show that Krueppel-Like Factor 5 Antibody, a BTEB2 Antibody, is suitable for flow cytometric analysis of KLF5 expression in human epithelial cells.