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Home >> Antibodies >> KRT77 Antibody / Keratin 77

KRT77 Antibody / Keratin 77 [clone MSVA-000R] (V6151)

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  Catalog No Formulation Size Price (USD)  
Image V6151-100UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 100 ug 599.50
Image
V6151-20UG Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide 20 ug 324.50
Microvalidated Recrabbitmono
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Keratin, type II cytoskeletal 1b Rabbit Recombinant Monoclonal Antibody (MSVA-000R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name MSVA-000R
UniProt Q7Z794 , Q01546
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This KRT77/Keratin 77 antibody is available for research use only.
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Application Notes

1. Optimal dilution of the KRT77/Keratin 77 antibody should be determined by the researcher.

2. This KRT77/Keratin 77 antibody is recombinantly produced by expression in human HEK293 cells.

Immunogen

A recombinant fragments and/or synthetic peptides of human Cytokeratin proteins (exact sequences are proprietary) was used as the immuongen for the KRT77/Keratin 77 antibody.

Storage

KRT77/Keratin 77 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.

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