KRT5 Antibody AGD-11 / Cytokeratin 5 Rabbit Monoclonal Antibody [clone AGD-11] (RQ5410)

KRT5 Antibody AGD-11 by IHC in Human Urothelial Carcinoma. Immunohistochemistry of FFPE human urothelial carcinoma tissue using KRT5 Antibody AGD-11. Strong brown cytoplasmic staining is observed throughout clusters of tumor cells, with relatively little staining in the surrounding stromal tissue. The Cytokeratin 5 Rabbit Monoclonal Antibody highlights the epithelial tumor architecture, while nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using 10 mM citrate buffer, pH 6, for 10-20 minutes.

KRT5 Antibody AGD-11 by WB in Human A431 Cells. Western blot analysis of human A431 cell lysate using KRT5 Antibody AGD-11. A prominent immunoreactive band is observed at approximately 60 kDa, consistent with the predicted molecular weight of cytokeratin 5 (58-62 kDa). The Cytokeratin 5 Rabbit Monoclonal Antibody demonstrates recognition of a protein migrating at the expected molecular weight of KRT5, supporting its use for investigating basal epithelial keratin expression.

KRT5 Antibody AGD-11 by WB in Human, Rat, and Mouse Samples. Western blot analysis using KRT5 Antibody AGD-11 with lysates from human A431 cells (lane 1), human HaCaT cells (lane 2), rat skin (lane 3), mouse skin (lane 4), and mouse thymus (lane 5). A prominent band is detected at approximately 60-65 kDa in all five samples, consistent with the predicted molecular weight of KRT5 (58-62 kDa). Additional higher molecular weight signals are visible in lanes 1 and 2. The Cytokeratin 5 Rabbit Monoclonal Antibody demonstrates reactivity across human, rat, and mouse samples.

KRT5 Antibody AGD-11 by IHC in Human Skin. Immunohistochemistry of FFPE human skin tissue using KRT5 Antibody AGD-11. Brown DAB staining is observed in epithelial cells surrounding hair follicle structures, with particularly prominent staining in the outer epithelial layers. The Cytokeratin 5 Rabbit Monoclonal Antibody highlights keratinocyte populations associated with epidermal appendages, while the surrounding connective tissue exhibits comparatively weak staining. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using EDTA buffer, pH 8, for 20 minutes.

KRT5 Antibody AGD-11 by IHC in Human Tonsil. Immunohistochemistry of FFPE human tonsil tissue using KRT5 Antibody AGD-11. Strong brown cytoplasmic staining is observed in the stratified squamous epithelium, contrasting with the predominantly unstained lymphoid tissue. The Cytokeratin 5 Rabbit Monoclonal Antibody highlights epithelial layers and their underlying architecture, while nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using EDTA buffer, pH 8, for 20 minutes.

KRT5 Antibody AGD-11 by IHC in Human Tonsil, Second View. Immunohistochemistry of FFPE human tonsil tissue using KRT5 Antibody AGD-11. Brown DAB staining highlights the squamous epithelial lining of a tonsillar crypt, with prominent staining in basal and suprabasal epithelial cells. The surrounding lymphoid tissue exhibits little staining, providing contrast with the epithelial compartment. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using EDTA buffer, pH 8, for 20 minutes.

KRT5 Antibody AGD-11 by IHC in Rat Skin. Immunohistochemistry of FFPE rat skin tissue using KRT5 Antibody AGD-11. Strong brown cytoplasmic staining is concentrated in the epithelial cells lining a hair follicle, with comparatively little staining in the surrounding connective tissue. The Cytokeratin 5 Rabbit Monoclonal Antibody demonstrates reactivity with rat KRT5 in follicular epithelial structures. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using EDTA buffer, pH 8, for 20 minutes.