KNG1 Antibody / Kininogen 1 Polyclonal Antibody (F54608)

NG1 Antibody by WB in Human Ramos Cells. Western blot analysis of human Ramos cell lysate using KNG1 Antibody. A distinct immunoreactive band is observed at approximately 70 kDa, consistent with the predicted molecular weight of approximately 72 kDa listed for kininogen 1. KNG1 encodes high molecular weight and low molecular weight kininogen, two plasma glycoproteins involved in inflammatory signaling and the kallikrein-kinin system. The observed band supports detection of a KNG1-related protein in human Ramos cells. Kininogen 1 Polyclonal Antibody recognizes a region shared by the major KNG1 isoforms, although the identity of the detected isoform has not been independently established.

KNG1 Antibody by WB in Transfected Human 293 Cells. Western blot analysis of non-transfected and KNG1-transfected human 293 cell lysates using KNG1 Antibody. Lane 1: non-transfected cells; lane 2: KNG1-transfected cells. A prominent immunoreactive band is detected at approximately 60 kDa in the transfected sample, while the corresponding signal is absent or substantially weaker in the non-transfected control. The observed molecular weight is below the approximately 72 kDa value listed on the datasheet. Differences in KNG1 processing, glycosylation, or construct characteristics could influence electrophoretic migration, although the specific explanation has not been established. The increased signal following transfection supports recognition of KNG1.

KNG1 Antibody by IHC in Human Breast Carcinoma. Immunohistochemistry of FFPE human breast carcinoma tissue using KNG1 Antibody. Brown DAB staining is observed predominantly in the cytoplasm of malignant epithelial cells, with variable staining intensity throughout the tissue. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer for 20 minutes, followed by cooling before antibody incubation. Because kininogen 1 is primarily a secreted plasma protein, tissue immunoreactivity may reflect local protein expression or the presence of circulating or deposited kininogen.

KNG1 Antibody by FACS in Human Ramos Cells. Flow cytometry analysis of human Ramos cells using KNG1 Antibody. The green histogram represents cells stained with KNG1 Antibody, while the blue histogram represents the isotype control. A pronounced rightward fluorescence shift is observed in the antibody-stained population relative to the control, demonstrating increased antibody-associated fluorescence. The distinct separation between the two populations supports detection of KNG1-associated immunoreactivity in Ramos cells. Because kininogen 1 is primarily a secreted protein, the fluorescence shift does not independently establish endogenous cell surface expression.