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Home >> Antibodies >> KMO Antibody / Kynurenine 3 monooxygenase

KMO Antibody / Kynurenine 3 monooxygenase (F54990)

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  Catalog No Formulation Size Price (USD)  
Image F54990-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54990-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human hepatocellular carcinoma tissue with KMO antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human hepatocellular carcinoma tissue with KMO antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human CCRF-CEM cell lysate with KMO antibody. Expected molecular weight: 52-56 kDa (multiple isoforms).
Western blot testing of mouse liver tissue lysate with KMO antibody. Expected molecular weight: 52-56 kDa (multiple isoforms).
Flow cytometry testing of human CCRF-CEM cells with KMO antibody; Blue=isotype control, Green= KMO antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt O15229
Localization Cytoplasmic
Applications Flow cytometry : 1:10-1:50 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:10-1:50
Western blot : 1:500-1:1000
Limitations This KMO antibody is available for research use only.
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Description

Kynurenine 3-monooxygenase (KMO) is an NADPH-dependent flavin monooxygenase that catalyzes the hydroxylation of the L-tryptophan metabolite L-kynurenine to form L-3-hydroxykynurenine.

Application Notes

The stated application concentrations are suggested starting points. Titration of the KMO antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 155-182 from the human protein was used as the immunogen for the KMO antibody.

Storage

Aliquot the KMO antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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