KLK7 Antibody / Kallikrein 7 [clone KLK7/4693] (V4640)

KLK7 Antibody immunohistochemistry analysis of Kallikrein 7 / KLK7 in human skin tissue. FFPE human skin tissue was stained with KLK7 antibody clone KLK7/4693 following heat induced epitope retrieval by boiling tissue sections in pH 9 Tris-EDTA buffer (10mM Tris, 1mM EDTA) for 20 minutes prior to cooling and staining. HRP-DAB brown chromogenic signal highlights strong staining in the outer epidermal layers near the stratum corneum, consistent with the known localization of Kallikrein 7 (KLK7), also referred to as stratum corneum chymotryptic enzyme, in differentiated keratinocytes involved in epidermal turnover and desquamation. Detection of KLK7 expression by immunohistochemistry supports studies of epidermal differentiation, keratinocyte biology, and skin barrier regulation using KLK7 Antibody / Kallikrein 7 (clone KLK7/4693).

IHC staining of FFPE human skin tissue with KLK7 antibody (clone KLK7/4693). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

KLK7 Antibody microarray specificity analysis of Kallikrein 7 using clone KLK7/4693. Analysis of a HuProt(TM) protein microarray containing more than 19,000 full length human proteins was performed with KLK7 antibody clone KLK7/4693 to evaluate target specificity across the human proteome. The antibody demonstrates strong and selective binding to KLK7 with the highest ranked signal intensity on the array. Results were generated using KLK7 Antibody / Kallikrein 7 (clone KLK7/4693), confirming selective recognition of the KLK7 protein relative to other proteins present on the array. Z-score represents the strength of signal produced when the monoclonal antibody, together with a fluorescently labeled anti-IgG secondary antibody, binds to a specific protein on the HuProt(TM) array and is expressed in units of standard deviations above the mean signal of the array. When proteins are ranked by Z-score, the S-score represents the difference between consecutive Z-scores and reflects the relative specificity of the antibody for its intended target. Antibodies with an S-score greater than 2.5 are considered highly specific, supporting selective recognition of KLK7 by clone KLK7/4693.