KLF5 Antibody / Epithelial Transcription Factor Antibody (FY13475)

KLF5 Antibody Human Colon Cancer IHC. Immunohistochemical analysis of FFPE human colon cancer tissue using KLF5 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody at 2 ug/mL overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Strong nuclear staining is observed in the neoplastic glandular epithelial cells, with minimal cytoplasmic immunoreactivity, consistent with the expected localization of the KLF5 transcription factor. These results demonstrate that KLF5 Antibody, an Epithelial Transcription Factor Antibody, specifically detects endogenous KLF5 in FFPE human colon cancer tissue.

KLF5 Antibody Mouse Small Intestine IHC. Immunohistochemical analysis of FFPE mouse small intestine tissue using KLF5 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody at 2 ug/mL overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Strong nuclear staining is observed predominantly within the proliferative epithelial cells of the intestinal crypts, with reduced staining toward the differentiated villus epithelium, consistent with the established role of KLF5 in epithelial proliferation and intestinal homeostasis. These results demonstrate that KLF5 Antibody, an Epithelial Transcription Factor Antibody, specifically detects endogenous KLF5 in FFPE mouse intestinal tissue.

KLF5 Antibody Rat Small Intestine High-Magnification IHC. Immunohistochemical analysis of FFPE rat small intestine tissue using KLF5 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody at 2 ug/mL overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. At higher magnification, strong nuclear staining is evident within the crypt epithelial cells, with comparatively weaker labeling of the differentiated villus epithelium, consistent with the established role of KLF5 in epithelial proliferation and intestinal homeostasis. These results demonstrate that KLF5 Antibody, an Epithelial Transcription Factor Antibody, specifically detects endogenous KLF5 in FFPE rat intestinal tissue.

KLF5 Antibody Rat Small Intestine IHC. Immunohistochemical analysis of FFPE rat small intestine tissue using KLF5 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody at 2 ug/mL overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Strong nuclear staining is observed predominantly within the epithelial cells of the intestinal crypts, with weaker staining in the differentiated villus epithelium, consistent with the established role of KLF5 in epithelial proliferation and intestinal homeostasis. These results demonstrate that KLF5 Antibody, an Epithelial Transcription Factor Antibody, specifically detects endogenous KLF5 in FFPE rat intestinal tissue.

KLF5 Antibody Human Stomach Cancer IHC. Immunohistochemical analysis of FFPE human stomach cancer tissue using KLF5 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody at 2 ug/mL overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Predominantly nuclear staining with variable cytoplasmic immunoreactivity is observed in the gastric carcinoma cells, consistent with the expected localization of the KLF5 transcription factor. These results demonstrate that KLF5 Antibody, an Epithelial Transcription Factor Antibody, specifically detects endogenous KLF5 in FFPE human cancer tissue.

KLF5 Antibody Human Skin Cancer IHC. Immunohistochemical analysis of FFPE human skin cancer tissue using KLF5 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody at 2 ug/mL overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Moderate nuclear staining with variable cytoplasmic immunoreactivity is observed throughout the tumor cells, consistent with the expected localization of the KLF5 transcription factor. These results demonstrate that KLF5 Antibody, an Epithelial Transcription Factor Antibody, specifically detects endogenous KLF5 in FFPE human cancer tissue.

KLF5 Antibody Rat Pancreas IHC. Immunohistochemical analysis of FFPE rat pancreas tissue using KLF5 Antibody. Heat-induced epitope retrieval was performed in pH 8.0 EDTA buffer prior to incubation with the primary antibody at 2 ug/mL overnight at 4°C. An HRP-conjugated goat anti-rabbit secondary antibody with DAB chromogen was used for visualization. Moderate nuclear staining with mild cytoplasmic immunoreactivity is observed in pancreatic epithelial cells, while the islets exhibit comparatively weaker staining, consistent with the expected localization of the KLF5 transcription factor. These results demonstrate that KLF5 Antibody, an Epithelial Transcription Factor Antibody, specifically detects endogenous KLF5 in FFPE rat pancreas tissue.

KLF5 Antibody SiHa Cell IF. Immunofluorescent staining of SiHa cells using KLF5 Antibody together with alpha tubulin antibody. Cells underwent enzyme-mediated antigen retrieval for 15 minutes before incubation with KLF5 Antibody at 5 ug/mL and an alpha tubulin antibody overnight at 4°C. A Cy3-conjugated goat anti-rabbit secondary antibody was used to detect KLF5 (red), while a Fluoro488-conjugated goat anti-mouse secondary antibody labeled alpha tubulin (green). Prominent nuclear localization of KLF5 is observed within the cytoskeletal framework defined by alpha tubulin, consistent with the expected distribution of this epithelial transcription factor. These results demonstrate that KLF5 Antibody, an Epithelial Transcription Factor Antibody, specifically detects endogenous KLF5 in cultured human cells.

KLF5 Antibody HeLa Cell IF. Immunofluorescent staining of HeLa cells using KLF5 Antibody together with alpha tubulin antibody. Cells underwent enzyme-mediated antigen retrieval for 15 minutes before incubation with KLF5 Antibody at 5 ug/mL and an alpha tubulin antibody overnight at 4°C. A Fluoro488-conjugated goat anti-rabbit secondary antibody was used to detect KLF5 (green), while a Cy3-conjugated goat anti-mouse secondary antibody labeled alpha tubulin (red). Prominent nuclear localization of KLF5 is observed against the cytoplasmic microtubule network, consistent with the expected distribution of this epithelial transcription factor. These results demonstrate that KLF5 Antibody, an Epithelial Transcription Factor Antibody, specifically detects endogenous KLF5 in cultured human cells.

KLF5 Antibody Human Cancer Cell WB. Western blot analysis of KLF5 Antibody using lysates from 1) human A549 cells, 2) human HeLa cells, 3) human Caco-2 cells, and 4) human PC-3 cells. Samples (30 ug/lane) were separated on a 10% SDS-PAGE gel under reducing conditions, transferred to a nitrocellulose membrane, and detected with KLF5 Antibody at 0.5 ug/mL followed by an HRP-conjugated secondary antibody and ECL detection. A specific band is observed at approximately 55 kDa in all cell lines, consistent with the expected molecular weight of KLF5 (approximately 51 kDa). These results demonstrate that KLF5 Antibody, an Epithelial Transcription Factor Antibody, specifically detects endogenous KLF5 across multiple human cancer cell lines.

KLF5 Antibody HeLa Cell IP. Immunoprecipitation of KLF5 from HeLa whole cell lysate using KLF5 Antibody, followed by Western blot detection with the same antibody. Lane 1: Input HeLa whole cell lysate (30 ug). Lane 2: Rabbit IgG control immunoprecipitation. Lane 3: KLF5 Antibody immunoprecipitation from 500 ug HeLa whole cell lysate using 2 ug of antibody. A specific band is detected at approximately 55 kDa, consistent with the expected migration of KLF5 (predicted molecular weight approximately 51 kDa). These results demonstrate that KLF5 Antibody, an Epithelial Transcription Factor Antibody, specifically immunoprecipitates endogenous KLF5 from human epithelial cells.