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Home >> Antibodies >> KIBRA Antibody / WWC1

KIBRA Antibody / WWC1 (RQ5851)

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  Catalog No Formulation Size Price (USD)  
Image RQ5851 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human A431 cells with KIBRA antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A431 cells with KIBRA antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) MCF7, 2) MDA-MB-453, 3) HEK293, 4) rat brain and 5) mouse brain lysate with KIBRA antibody. Predicted molecular weight ~125 kDa.
Flow cytometry testing of human A431 cells with KIBRA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KIBRA antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q8IX03
Localization Cytoplasmic, nuclear, plasma membrane
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This KIBRA antibody is available for research use only.
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Description

Protein KIBRA also known as 'kidney and brain expressed protein' or WW domain-containing protein 1 (WWC1) is a protein that in humans is encoded by the WWC1 gene. The protein encoded by this gene is a cytoplasmic phosphoprotein that interacts with PRKC-zeta and dynein light chain-1. Alleles of this gene have been found that enhance memory in some individuals. Three transcript variants encoding different isoforms have been found for this gene.

Application Notes

Optimal dilution of the KIBRA antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids D990-Q1080) was used as the immunogen for the KIBRA antibody.

Storage

After reconstitution, the KIBRA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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