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Home >> Antibodies >> Ki67 Antibody

Ki67 Antibody (R31458)

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  Catalog No Formulation Size Price (USD)  
Image R31458 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of Ki67 antibody and human samples 1: HeLa; 2: MCF-7; 3: COLO320; 4: HEPG2; 5: SKOV lysate. Predicted molecular weight ~350 kDa.
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Western blot testing of Ki67 antibody and human samples 1: HeLa; 2: MCF-7; 3: COLO320; 4: HEPG2; 5: SKOV lysate. Predicted molecular weight ~350 kDa.
IHC-P: Ki67 antibody testing of FFPE human tonsil tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human colon tissue with Ki67 antibody (green) at 5ug/ml and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Immunocytochemical staining of FFPE human HeLa cells with Ki67 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose
Gene ID 4288
Localization Nuclear
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Immunocytochemistry : 0.5-1ug/ml
Limitations This Ki67 antibody is available for research use only.
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Description

Proliferation-related Ki-67 antigen, also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Ki67 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 2860-3256) was used as the immunogen for this Ki67 antibody.

Storage

After reconstitution, the Ki67 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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