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Home >> Antibodies >> Ki67 Antibody

Ki67 Antibody [clone 5C7] (RQ7913)

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  Catalog No Formulation Size Price (USD)  
Image RQ7913 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
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Immunofluorescent staining of FFPE human A431 cells with Ki67 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human esophageal squamous carcinoma tissue with Ki67 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cervical cancer tissue with Ki67 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with Ki67 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymphoma tissue with Ki67 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with Ki67 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human Jurkat cells with Ki67 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ki67 antibody.
Western blot testing of human K562 cell lysate with Ki67 antibody. Predicted molecular weight ~350 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 5C7
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P46013
Localization Nucleus, Nucleolus
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow Cytometry : 1-3ug/million cells
Limitations This Ki67 antibody is available for research use only.
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Description

Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.

Application Notes

Optimal dilution of the Ki67 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids K2860-I3256) was used as the immunogen for the Ki67 antibody.

Storage

After reconstitution, the Ki67 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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