Ki-67 Antibody Biotin Conjugate / MKI67 Biotinylated Antibody [clone MKI67/2465] (V3866BTN)

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Ki-67 antibody (clone MKI67/2465). These results demonstrate the foremost specificity of the MKI67/2465 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Ki-67 Antibody Biotin Conjugate / MKI67 Biotinylated Antibody (clone MKI67/2465). Immunohistochemistry analysis of FFPE human tonsil tissue using Ki-67 Antibody Biotin Conjugate (clone MKI67/2465). Strong nuclear brown staining is observed in numerous proliferating lymphoid cells within the germinal center region of the tonsil, consistent with the known expression pattern of Ki-67 / MKI67 in actively dividing cells. The biotin-conjugated antibody format enables detection through avidin-biotin based staining systems, producing clear nuclear labeling of proliferating lymphocytes within the lymphoid follicle. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 minutes followed by cooling prior to staining.

SDS-PAGE analysis of purified, BSA-free Ki-67 antibody (clone MKI67/2465) as confirmation of integrity and purity.