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Home >> Antibodies >> Ki-67 Antibody

Ki-67 Antibody [clone MKI67/2465] (V3866)

  Catalog No Formulation Size Price (USD)  
Image V3866-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 499
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V3866-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 219
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V3866SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 499
IHC testing of FFPE human tonsil stained with Ki-67 antibody (MKI67/2465). Required HIER: boiling tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Ki-67 antibody (clone MKI67/2465). These results demonstrate the foremost specificity of the MKI67/2465 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
FACS testing of human HeLa cells with Ki-67 antibody (blue, clone MKI67/2465) and isotype control (red). Cells were trypsinized and 2-4% PFA-fixed prior to staining.
Immunofluorescent staining of human HeLa cells with Ki-67 antibody (green, clone MKI67/2465) and Phalloidin (red, membrane stain).
SDS-PAGE analysis of purified, BSA-free Ki-67 antibody (clone MKI67/2465) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name MKI67/2465
Purity Protein G affinity chromatography
UniProt P46013
Localization Nuclear
Applications Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Ki-67 antibody is available for research use only.
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Description

Ki67 antigen is a nuclear, non-histone protein that is present in all stages of the cell cycle except G0. This characteristic makes Ki67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. A correlation has been demonstrated between Ki67 index and the histo-pathological grade of neoplasms. Assessment of Ki67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors. Ki67 labeling index has been shown to be a prognostic marker in a number of neoplasms including grade II astrocytoma, oligodendroglioma, colon carcinoma, and breast carcinoma. In general, Ki67 is a good marker of proliferating cell populations.

Application Notes

Optimal dilution of the Ki-67 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 2293-2478 was used as the immunogen for the Ki-67 antibody.

Storage

Store the Ki-67 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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