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Home >> Antibodies >> Keratin 8 Antibody / Cytokeratin 8

Keratin 8 Antibody / Cytokeratin 8 [clone CIG-11] (RQ5132)

  Catalog No Formulation Size Price (USD)  
Image RQ5132 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 429
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Western blot testing of human HaCat cell lysate with Keratin 8 antibody. Predicted molecular weight ~56 kDa.
IHC staining of FFPE human human liver with Keratin 8 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Availability 1-2 weeks
Species Reactivity Human
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name CIG-11
Purity Affinity purified
UniProt P05787
Applications Western blot : 1:10000-1:20000
Immunohistochemistry (FFPE) : 1:50-1:200
Limitations This Keratin 8 antibody is available for research use only.
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Description

The KRT8 gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [RefSeq]

Application Notes

Optimal dilution of the Keratin 8 antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human Cytokeratin 8 / KRT8 was used as the immunogen for the Keratin 8 antibody.

Storage

Store the Keratin 8 antibody at -20oC.

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