Keratin 8 Antibody / Cytokeratin 8 / CK8 [clone KRT8/803] (V2664)

Description

Keratin 8 antibody is useful for studying Keratin 8, also known as Cytokeratin 8 (CK8 or KRT8), a type II intermediate filament protein that forms an essential component of the cytoskeletal network in simple epithelial cells. Cytokeratin 8 typically pairs with Cytokeratin 18 to create intermediate filaments that provide structural integrity, mechanical stability, and resistance to cellular stress. As one of the most widely expressed epithelial cytokeratins, Cytokeratin 8 serves as an important marker for epithelial cell identification, differentiation, and tumor characterization in both normal tissues and neoplastic lesions.

Cytokeratin 8 is expressed in a broad range of simple epithelial tissues, including glandular epithelium, gastrointestinal tract, respiratory epithelium, liver, pancreas, and many secretory organs. The protein contributes to maintenance of cellular architecture while also participating in signaling pathways that regulate cell growth, apoptosis, migration, and stress responses. In addition to its structural role, Cytokeratin 8 interacts with numerous intracellular proteins involved in signal transduction and cytoprotection, highlighting its importance beyond cytoskeletal support alone.

Altered Cytokeratin 8 expression has been associated with epithelial injury, tissue remodeling, inflammation, and cancer progression. Changes in KRT8 expression patterns are frequently observed during epithelial transformation and tumor development, making Cytokeratin 8 a valuable biomarker in cancer research and diagnostic pathology. Antibodies targeting Cytokeratin 8 are commonly used to identify epithelial-derived tumors and to distinguish carcinomas from tumors of mesenchymal, lymphoid, or neuroectodermal origin.

Keratin 8 is expressed in a broad range of simple epithelial tissues, including glandular epithelium, gastrointestinal tract, respiratory epithelium, liver, pancreas, and many secretory organs. The protein contributes to maintenance of cellular architecture while also participating in signaling pathways that regulate cell growth, apoptosis, migration, and stress responses. In addition to its structural role, Keratin 8 interacts with numerous intracellular proteins involved in signal transduction and cytoprotection, highlighting its importance beyond cytoskeletal support alone.

Altered Keratin 8 expression has been associated with epithelial injury, tissue remodeling, inflammation, and cancer progression. Changes in KRT8 expression patterns are frequently observed during epithelial transformation and tumor development, making Keratin 8 a valuable biomarker in cancer research and diagnostic pathology. Antibodies targeting Keratin 8 are commonly used to identify epithelial-derived tumors and to distinguish carcinomas from tumors of mesenchymal, lymphoid, or neuroectodermal origin.

Because Keratin 8 is broadly expressed across many epithelial cell types, it is frequently included in antibody panels used for tissue classification, tumor characterization, and studies of epithelial differentiation. KRT8 expression is often evaluated alongside other cytokeratins to assess epithelial lineage, tissue origin, and phenotypic changes associated with development or disease progression. The protein is particularly valuable for investigating simple epithelial biology and epithelial-associated malignancies.

Keratin 8 antibodies are widely utilized in immunohistochemistry, western blotting, immunofluorescence, flow cytometry, and related protein detection applications. These reagents support studies of epithelial cell biology, intermediate filament organization, cancer pathology, tissue differentiation, and cellular stress responses. Keratin 8 antibody tools help researchers characterize epithelial populations, evaluate tumor-associated expression patterns, and investigate the biologic functions of this important cytoskeletal protein.

Researchers seeking protein microarray validated detection of Keratin 8 for epithelial differentiation, cytoskeletal biology, and cancer research applications may also be interested in our Cytokeratin 8 Antibody page.

Application Notes

Optimal dilution of the Keratin 8 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human KRT8 protein was used as the immunogen for the Keratin 8 antibody.

Storage

Store the Keratin 8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Cytokeratin 8 Antibody, CK8 Antibody, KRT8 Antibody, Keratin Type II Cytoskeletal 8 Antibody, Simple Epithelial Keratin Antibody, Intermediate Filament Protein 8 Antibody