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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Keratin 20 Antibody for IF / Cytokeratin 20 KRT20 Immunofluorescence Antibody (RQ5632)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RQ5632 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 449 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Purity | Affinity purified |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide |
| UniProt | Q9D312 |
| Localization | Cytoplasmic |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry : 1-2ug/ml Immunofluorescence : 2-4ug/ml Flow Cytometry : 1-3ug/million cells Direct ELISA : 0.1-0.5ug/ml |
| Limitations | This Keratin 20 antibody is available for research use only. |
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Keratin 20 (KRT20) is a type I acidic cytokeratin that belongs to the intermediate filament protein family responsible for maintaining epithelial cell structure and mechanical stability. Keratin 20 is primarily localized to the cytoplasm of epithelial cells, where it forms filament networks together with type II keratins to support cytoskeletal organization and epithelial integrity. Keratin 20 Antibody for IF enables sensitive visualization of cytokeratin filament organization and epithelial cell identity using immunofluorescence microscopy. KRT20 expression is strongly associated with gastrointestinal epithelial differentiation and is commonly detected in intestinal mucosa, gastric epithelium, and urothelial umbrella cells, making it a widely used epithelial lineage marker in research.
Keratin 20 is encoded by the KRT20 gene located on chromosome 17q21 and is part of the large keratin gene cluster that includes multiple epithelial cytokeratin family members. The Keratin 20 protein, also widely referred to as Cytokeratin 20 or CK20 in the literature, forms heterodimeric complexes with type II keratins such as keratin 8. These heterodimers polymerize to create intermediate filament structures that extend throughout the cytoplasm, providing mechanical resilience to epithelial tissues exposed to constant mechanical stress. Keratin 20 Antibody for IF is therefore useful for visualizing these filamentous cytoskeletal networks within epithelial cells when examined by immunofluorescence imaging.
Within normal tissues, Keratin 20 expression is characteristically restricted to differentiated epithelial populations, particularly intestinal epithelial cells and urothelial umbrella cells. This restricted distribution has made Cytokeratin 20 antibody reagents valuable tools for studying epithelial differentiation pathways and for distinguishing intestinal epithelial lineage cells in tissue sections and cultured models. Immunofluorescence staining using a Keratin 20 Antibody for IF typically reveals a filamentous cytoplasmic staining pattern that corresponds to the intermediate filament network distributed throughout epithelial cell cytoplasm. In polarized epithelial tissues, CK20 expression can highlight specific differentiated cell layers, particularly in gastrointestinal mucosa.
In cancer biology, Keratin 20 has significant relevance because its expression pattern is frequently retained in epithelial tumors derived from gastrointestinal and urothelial tissues. Cytokeratin 20 antibody detection is commonly used in studies of colorectal carcinoma, gastric carcinoma, pancreatic carcinoma, and bladder cancer, where KRT20 expression often reflects the differentiation state of tumor epithelial cells. Immunofluorescence imaging with Keratin 20 Antibody for IF allows researchers to visualize cytoskeletal organization and epithelial lineage markers in tumor samples, organoids, and cultured cancer cell models. The characteristic cytoplasmic filament pattern observed with Cytokeratin 20 antibody staining helps distinguish epithelial tumor cells from surrounding stromal or immune populations.
Beyond oncology research, Keratin 20 also plays a role in epithelial cell maturation and structural organization in the intestinal tract. Studies have shown that CK20 expression increases during epithelial differentiation as cells migrate along the crypt-villus axis, highlighting its association with mature epithelial phenotypes. Keratin 20 Antibody for IF is therefore widely used in studies of intestinal epithelial development, epithelial polarity, and barrier function. Because immunofluorescence microscopy provides high spatial resolution, Cytokeratin 20 antibody staining is particularly useful for evaluating subcellular cytoskeletal architecture and epithelial cell morphology within complex tissue environments.
A rabbit polyclonal Keratin 20 Antibody for IF can be used to detect Keratin 20 protein distribution in fixed cells and tissue sections for research applications involving epithelial biology, cytoskeletal organization, and epithelial lineage identification. When applied in immunofluorescence experiments, KRT20 antibody labeling typically reveals strong cytoplasmic filament staining in epithelial cells, enabling visualization of epithelial structural networks and aiding studies of epithelial differentiation and tumor biology.
Optimal dilution of the Keratin 20 Antibody for IF should be determined by the researcher.
Recombinant mouse protein (amino acids R29-V431) was used as the immunogen for the Keratin 20 antibody.
After reconstitution, the Keratin 20 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Cytokeratin 20 antibody, CK20 antibody, Keratin 20 antibody, KRT20 antibody, Cytokeratin-20 antibody
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