Keratin 19 Antibody 28K37 / KRT19 Rabbit Monoclonal Antibody [clone 28K37] (RQ8356)

Keratin 19 Antibody 28K37 by WB in human HepG2 cells. Western blot analysis of human HepG2 cell lysate using Keratin 19 Antibody clone 28K37. A prominent immunoreactive band is detected at approximately 43 kDa, consistent with the predicted molecular weight of human KRT19. The band migrates between the 37 and 50 kDa molecular weight markers, closer to the 50 kDa position. The displayed lane exhibits a concentrated signal with relatively little background staining, and no prominent additional bands are apparent. The KRT19 Rabbit Monoclonal Antibody demonstrates recognition of a protein migrating near the expected molecular weight of cytokeratin 19 in this human liver-derived carcinoma cell model.

Keratin 19 Antibody 28K37 by WB in human HepG2 and MCF7 cells. Western blot analysis of human HepG2 (lane 1) and MCF7 (lane 2) cell lysates using Keratin 19 Antibody clone 28K37. Both lanes exhibit prominent immunoreactive bands near 43 kDa, consistent with the predicted molecular weight of KRT19. The signal in MCF7 lysate is substantially stronger and broader than that observed in HepG2 lysate, with additional staining extending toward higher molecular weights. This difference may reflect variation in sample loading, protein abundance, or electrophoretic behavior, although the underlying cause cannot be determined from the image. The KRT19 Rabbit Monoclonal Antibody demonstrates immunoreactivity in both human carcinoma cell lines.

Keratin 19 Antibody 28K37 by WB in human SK-BR-3 cells. Western blot analysis of human SK-BR-3 breast carcinoma cell lysate using Keratin 19 Antibody clone 28K37. A prominent immunoreactive band is visible at approximately 43-45 kDa, migrating between the 40 and 55 kDa molecular weight markers. This position is consistent with the expected molecular weight of human cytokeratin 19, approximately 43 kDa. An additional, weaker band is visible near 35 kDa. The identity of this lower molecular weight signal has not been established, and it cannot be assigned to a particular KRT19 fragment or isoform without further validation. The KRT19 Rabbit Monoclonal Antibody demonstrates recognition of a protein migrating near the expected size of KRT19.

Keratin 19 Antibody 28K37 by WB in human MCF7 and RT4 cells. Western blot analysis of human MCF7 breast carcinoma (lane 1) and RT4 bladder carcinoma (lane 2) cell lysates using Keratin 19 Antibody clone 28K37. Both lanes exhibit prominent immunoreactive bands migrating at approximately 43 kDa, consistent with the predicted molecular weight of KRT19. The bands appear similar in position, with the RT4 sample showing slightly greater signal intensity. No prominent additional immunoreactive bands are visible within the displayed molecular weight range. The KRT19 Rabbit Monoclonal Antibody demonstrates recognition of a protein migrating near the expected size of cytokeratin 19 in two epithelial carcinoma cell models.

Keratin 19 Antibody 28K37 by IHC in human breast cancer. Immunohistochemistry of formalin-fixed, paraffin-embedded human breast cancer tissue using Keratin 19 Antibody clone 28K37. Brown immunoreactivity highlights numerous irregular glandular and epithelial tumor structures distributed throughout the tissue section. The staining is predominantly associated with the cytoplasmic compartment of carcinoma cells, with variable intensity among adjacent epithelial formations. Surrounding stromal regions exhibit comparatively limited immunoreactivity, while hematoxylin counterstaining reveals nuclei in blue. The KRT19 Rabbit Monoclonal Antibody demonstrates cytokeratin 19 expression within the neoplastic epithelial compartment. Heat-induced epitope retrieval was performed by boiling tissue sections in EDTA buffer, pH 8, for 20 minutes, followed by cooling before antibody incubation.

Keratin 19 Antibody 28K37 by IHC in human appendix. Immunohistochemistry of formalin-fixed, paraffin-embedded human appendix tissue using Keratin 19 Antibody clone 28K37. Brown cytoplasmic immunoreactivity highlights glandular epithelial cells lining rounded and elongated intestinal crypt structures. The staining is particularly apparent along the epithelial boundaries of the glandular lumens, while surrounding connective tissue and inflammatory cell populations exhibit comparatively limited immunoreactivity. Hematoxylin counterstaining reveals nuclei in blue. The KRT19 Rabbit Monoclonal Antibody demonstrates the distribution of cytokeratin 19 within appendiceal glandular epithelium. Heat-induced epitope retrieval was performed by boiling tissue sections in EDTA buffer, pH 8, for 20 minutes, followed by cooling before antibody incubation.

Keratin 19 Antibody 28K37 by IHC in rat small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded rat small intestine tissue using Keratin 19 Antibody clone 28K37. Brown immunoreactivity is distributed along intestinal epithelial structures, with staining visible in portions of the mucosal epithelium and glandular regions. The positively stained epithelial cells contrast with the surrounding lamina propria, where immunoreactivity is comparatively limited. Hematoxylin counterstaining reveals nuclei in blue. The KRT19 Rabbit Monoclonal Antibody demonstrates recognition of keratin 19-associated epithelial structures in rat intestinal tissue, supporting the documented rat reactivity. Heat-induced epitope retrieval was performed by boiling tissue sections in EDTA buffer, pH 8, for 20 minutes, followed by cooling before antibody incubation.