Keratin 15 Antibody LHK15 / Cytokeratin 15 Monoclonal Antibody [clone LHK15] (V3286)

Keratin 15 Antibody LHK15 by IHC in human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded human skin tissue using the Cytokeratin 15 Monoclonal Antibody. Brown immunoreactivity is concentrated within a narrow epithelial region bordering the underlying connective tissue, with staining predominantly associated with the cytoplasm of basal epithelial cells. The adjacent tissue exhibits comparatively limited labeling, while hematoxylin counterstaining highlights nuclei in blue. The distribution is consistent with the basal epithelial localization associated with keratin 15 expression. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM citrate buffer, pH 6, for 10-20 minutes.

SDS-PAGE analysis of purified Keratin 15 Antibody LHK15. Electrophoretic analysis of the purified, BSA-free Cytokeratin 15 Monoclonal Antibody under nonreducing (NR) and reducing (R) conditions, with approximately 2 ug of antibody loaded per lane. The nonreducing lane contains a prominent band migrating near 150 kDa, consistent with intact immunoglobulin. Under reducing conditions, distinct bands are observed near 50 kDa and 25 kDa, corresponding to immunoglobulin heavy and light chains, respectively. The observed migration patterns are consistent with the expected molecular composition of a purified mouse monoclonal antibody and support the integrity of the antibody preparation.