Keratin 15 Antibody KRT15/2958 / Cytokeratin 15 Monoclonal Antibody [clone KRT15/2958] (V7920)

Keratin 15 Antibody KRT15/2958 by WB in human thymus tissue. Western blot analysis of human thymus tissue lysate using the Cytokeratin 15 Monoclonal Antibody. A prominent immunoreactive band is detected at approximately 50 kDa, consistent with the predicted molecular weight of human Keratin 15. The band is well defined, with minimal background signal and no additional prominent bands visible across the molecular weight range shown. KRT15 is a type I intermediate filament protein associated with basal epithelial cells and specialized epithelial compartments. The observed migration supports detection of a protein corresponding to the expected molecular weight of human Cytokeratin 15.

Keratin 15 Antibody KRT15/2958 by FACS in human HeLa cells. Flow cytometry analysis of paraformaldehyde-fixed HeLa human cervical carcinoma cells using the Cytokeratin 15 Monoclonal Antibody. The cyan histogram represents antibody staining, while the red histogram represents the isotype control. A pronounced rightward fluorescence shift is observed in the antibody-stained population, with relatively little overlap between the two distributions. This separation demonstrates increased antibody-associated fluorescence compared with the control, supporting detection of intracellular KRT15 in the examined cell population. The distinct fluorescence profiles indicate recognition of the target under the experimental conditions.

Keratin 15 Antibody KRT15/2958 by IHC in human prostate carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human prostate carcinoma tissue using the Cytokeratin 15 Monoclonal Antibody. Strong brown cytoplasmic staining is concentrated within epithelial cells lining irregularly shaped glandular structures, with particularly prominent labeling along the glandular boundaries. The surrounding stromal tissue exhibits comparatively limited immunoreactivity, while hematoxylin counterstaining highlights nuclei in blue. The staining distribution demonstrates KRT15 expression in selected epithelial compartments of the examined tissue. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 minutes.

Keratin 15 Antibody KRT15/2958 by IHC in human prostate carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded human prostate carcinoma tissue using the Cytokeratin 15 Monoclonal Antibody. Brown immunoreactivity is visible primarily along the epithelial lining of scattered glandular structures, with a more restricted and discontinuous staining pattern than in the preceding image. Large areas of surrounding tissue exhibit little detectable staining, while hematoxylin counterstaining highlights nuclei and stromal architecture in blue. The distribution illustrates heterogeneous KRT15 immunoreactivity among epithelial structures within the examined section. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 10-20 minutes.

Keratin 15 Antibody KRT15/2958 by protein microarray analysis. Protein microarray specificity assessment of the Cytokeratin 15 Monoclonal Antibody using a HuProt array containing more than 19,000 full-length human proteins. KRT15 produces the highest-ranking signal, with a Z-score of approximately 51.2, followed by substantially weaker interactions with KRT13 and HLA-A. The pronounced separation between KRT15 and the remaining proteins supports preferential recognition of the intended target under the assay conditions. Z-scores represent signal strength relative to the array mean, while S-scores describe differences between ranked signals. These findings support the designation Protein Microarray Validated.