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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
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Keratin 10 antibody recognizes Cytokeratin 10, a type I intermediate filament protein encoded by the KRT10 gene that serves as a hallmark of suprabasal keratinocyte differentiation in stratified squamous epithelia. Keratin 10 is induced as keratinocytes withdraw from the cell cycle and transition from the basal proliferative compartment into differentiated suprabasal layers. In this context, Keratin 10 pairs primarily with Keratin 1 to assemble intermediate filament networks that provide mechanical strength, structural integrity, and resilience to the epidermis. Because of this tightly regulated expression pattern, KRT10 antibody is widely used to study epidermal differentiation, epithelial maturation, and keratinocyte lineage commitment.
In normal tissues, Keratin 10 expression is largely restricted to the suprabasal layers of the epidermis and other stratified squamous epithelia, where it displays a characteristic cytoplasmic filamentous staining pattern. Basal keratinocytes typically lack Keratin 10 expression, instead expressing basal keratins such as Keratin 5 and Keratin 14. This clear compartmentalization makes KRT10 antibody a valuable tool for distinguishing differentiated suprabasal cells from proliferative basal cells in both normal and diseased tissue. Keratin 10 expression therefore reflects the functional state of keratinocytes and the integrity of epidermal stratification.
Keratin 10 has important relevance in dermatopathology and epithelial tumor biology. Altered or reduced Cytokeratin 10 expression is frequently observed in hyperproliferative skin disorders, inflammatory dermatoses, and conditions associated with impaired differentiation. In squamous cell carcinoma and related epithelial malignancies, KRT10 antibody staining can assist in evaluating the degree of tumor differentiation, as well-differentiated lesions are more likely to retain Keratin 10 expression compared with poorly differentiated tumors. Genetic disorders of keratinization, including epidermolytic hyperkeratosis, further underscore the functional importance of Keratin 10 in maintaining epidermal stability.
At the molecular level, Keratin 10 belongs to the acidic type I keratin family and contains conserved alpha-helical domains required for heterodimer formation and filament assembly. Its expression is regulated by differentiation-associated transcriptional programs and calcium-dependent signaling pathways. A KRT10 antibody therefore provides a robust reagent for investigating epidermal biology, epithelial differentiation status, and disease-associated alterations in stratified squamous epithelia.
Optimal dilution of the Keratin 10 antibody should be determined by the researcher.
Recombinant full-length human KRT10 protein was used as the immunogen for the Keratin 10 antibody.
Aliquot the Keratin 10 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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