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Home >> Antibodies >> KAP1 Antibody / TRIM28

KAP1 Antibody / TRIM28 (F54620)

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  Catalog No Formulation Size Price (USD)  
Image F54620-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
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F54620-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of human 1) HeLa and 2) PC-3 cell lysate with KAP1 antibody. Expectd molecular weight: 88~110 kDa depending on sumoylation level.
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Western blot testing of human 1) HeLa and 2) PC-3 cell lysate with KAP1 antibody. Expectd molecular weight: 88~110 kDa depending on sumoylation level.
Western blot testing of human K562 cell lysate with KAP1 antibody. Expectd molecular weight: 88~110 kDa depending on sumoylation level.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with KAP1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
IHC testing of FFPE human testis tissue with KAP1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q13263
Localization Nuclear
Applications Immunofluorescence : 1:25
Immunohistochemistry (FFPE) : 1:25
Western blot : 1:500-1:2000
Limitations This KAP1 antibody is available for research use only.
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Description

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Application Notes

The stated application concentrations are suggested starting points. Titration of the KAP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 178-207 from the human protein was used as the immunogen for the KAP1 antibody.

Storage

Aliquot the KAP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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