Kallikrein 7 Antibody / KLK7 [clone KLK7/4692] (V4650)

Kallikrein 7 Antibody immunohistochemistry analysis of KLK7 in human skin tissue using clone KLK7/4692. FFPE human skin tissue was stained with Kallikrein 7 antibody clone KLK7/4692 following heat induced epitope retrieval by boiling tissue sections in pH 9 Tris-EDTA buffer (10mM Tris, 1mM EDTA) for 20 minutes prior to cooling and staining. HRP-DAB brown chromogenic signal highlights strong staining along the outer epidermal layers near the stratum corneum, consistent with the known localization of Kallikrein 7 (KLK7), also known as stratum corneum chymotryptic enzyme, in differentiated keratinocytes involved in epidermal turnover and desquamation. Detection of KLK7 expression by immunohistochemistry supports studies of epidermal differentiation and keratinocyte biology using Kallikrein 7 Antibody / KLK7 (clone KLK7/4692).

SDS-PAGE analysis of purified, BSA-free Kallikrein 7 antibody (clone KLK7/4692) as confirmation of integrity and purity.

KLK7 Antibody protein microarray specificity analysis using Kallikrein 7 antibody clone KLK7/4692. A HuProt(TM) protein microarray containing more than 19,000 full-length human proteins was probed using KLK7 antibody clone KLK7/4692 followed by a fluorescently labeled anti-IgG secondary antibody to evaluate target specificity. Signal strength is represented by the Z-score, which reflects the number of standard deviations above the mean signal intensity across the entire array. Targets are ranked by descending Z-score to identify the strongest antibody-protein interactions. The S-score represents the difference in Z-scores between the top-ranked protein and the next highest signal, providing a measure of target specificity. In this analysis, KLK7 produced the highest Z-score and a strong S-score separation relative to other detected proteins, indicating highly specific binding of the antibody to Kallikrein 7 (KLK7). These results support the specificity of Kallikrein 7 Antibody / KLK7 (clone KLK7/4692) for detecting KLK7 in proteomic and immunodetection studies.

KLK7 Antibody for WB western blot analysis of Kallikrein 7 in human tissue lysates. Lane 1: human liver lysate, Lane 2: human skin lysate. Samples were resolved by SDS-PAGE and probed with Kallikrein 7 antibody clone KLK7/4692 followed by detection with an appropriate secondary antibody. A band is detected at approximately 28-30 kDa, consistent with the predicted molecular weight of Kallikrein 7 (KLK7), a secreted serine protease also known as stratum corneum chymotryptic enzyme that is primarily expressed in epidermal keratinocytes. The stronger signal observed in human skin lysate aligns with the known enrichment of KLK7 in epidermal tissue involved in skin barrier function and desquamation. Detection of KLK7 expression supports use of Kallikrein 7 Antibody / KLK7 (clone KLK7/4692) for western blot analysis of KLK7 protein in human tissue samples.