Transcription Factor JunB Antibody / JUNB Polyclonal Antibody (RQ6565)

Transcription Factor JunB Antibody Shows Nuclear Staining in Human Spleen IHC. Immunohistochemistry staining of FFPE human spleen tissue with Transcription Factor JunB Antibody. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. The image shows widespread nuclear brown staining among cells throughout the splenic tissue, with variable staining intensity and comparatively little cytoplasmic signal. The predominantly nuclear distribution is consistent with the function of JunB as an AP-1 transcription factor and demonstrates tissue detection using this JUNB Polyclonal Antibody.

Transcription Factor JunB Antibody Highlights Placental Trophoblasts in Human Placenta IHC. Immunohistochemistry staining of FFPE human placental tissue with Transcription Factor JunB Antibody. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. Brown JUNB staining is present within cells lining the chorionic villi, with prominent nuclear labeling and comparatively weak signal in the villous stromal compartments. The distinct trophoblast-associated staining demonstrates tissue detection using this JUNB Polyclonal Antibody.

Transcription Factor JunB Antibody in Human Gall Bladder Adenosquamous Carcinoma IHC. Immunohistochemistry staining of FFPE human gall bladder adenosquamous carcinoma tissue with Transcription Factor JunB Antibody. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. The image shows prominent brown nuclear staining in the malignant epithelial cells surrounding a glandular lumen, with variable nuclear signal also present in adjacent cells. The predominantly nuclear pattern is consistent with the transcriptional function of JunB and demonstrates tumor tissue detection using this JUNB Polyclonal Antibody.

Transcription Factor JunB Antibody Shows Nuclear Labeling in Human Lung Cancer IHC. Immunohistochemistry staining of FFPE human lung cancer tissue with Transcription Factor JunB Antibody. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. Numerous tumor cells exhibit brown nuclear JUNB staining distributed throughout the malignant tissue, with heterogeneous signal intensity across the field and relatively limited cytoplasmic staining. The predominantly nuclear pattern is consistent with JunB's role as an AP-1 transcription factor and demonstrates tumor tissue detection using this JUNB Polyclonal Antibody.

Transcription Factor JunB Antibody in Human Pancreatic Cancer IHC. Immunohistochemistry staining of FFPE human pancreatic cancer tissue with Transcription Factor JunB Antibody. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. The image shows widespread brown staining among the malignant epithelial cells, with prominent nuclear labeling accompanied by some cytoplasmic signal. Staining intensity varies across the tumor population, while intervening stromal areas show comparatively less labeling. The prominent nuclear component is consistent with JunB localization as an AP-1 transcription factor and demonstrates tissue detection using this JUNB Polyclonal Antibody.

Transcription Factor JunB Antibody Shows Widespread Nuclear Staining in Mouse Brain IHC. Immunohistochemistry staining of FFPE mouse brain tissue with Transcription Factor JunB Antibody. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. Numerous nuclei throughout the field display brown JUNB labeling, producing a widespread punctate nuclear staining pattern with comparatively little diffuse cytoplasmic signal. The result demonstrates recognition of endogenous mouse JunB and supports the cross-species utility of this JUNB Polyclonal Antibody.

Transcription Factor JunB Antibody Demonstrates Nuclear Staining in Rat Brain IHC. Immunohistochemistry staining of FFPE rat brain tissue with Transcription Factor JunB Antibody. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. Numerous nuclei show strong, discrete brown labeling, with differences in the density of positive cells across the brain tissue and comparatively little diffuse cytoplasmic staining. The nuclear distribution supports detection of endogenous rat JunB and demonstrates cross-species reactivity of this JUNB Polyclonal Antibody.

Transcription Factor JunB Antibody Shows Nuclear Localization in SiHa Cervical Cancer Cells IF. Immunofluorescence staining of FFPE human SiHa cervical cancer cells with Transcription Factor JunB Antibody (green) and DAPI nuclear stain (blue). HIER was performed by steaming in pH 6 citrate buffer for 20 min. Comparison of the DAPI and antibody panels shows strong green JUNB signal corresponding closely with the nuclei across the cell population, with comparatively little extranuclear fluorescence. The predominantly nuclear distribution is consistent with the transcriptional role of JunB and demonstrates cellular detection using this JUNB Polyclonal Antibody.

Transcription Factor JunB Antibody Western Blot of Multiple Human Cell Lines. Western blot testing of human HeLa (lane 1), K562 (lane 2), Caco-2 (lane 3), A549 (lane 4), HT1080 (lane 5), and A431 (lane 6) cell lysates with Transcription Factor JunB Antibody. JUNB-reactive bands are detected at approximately 40-45 kDa across all six lysates, with differences in signal intensity between samples and particularly strong signal in A549 cells. The migration is consistent with phosphorylated JUNB, which can appear above the approximately 36-39 kDa non-phosphorylated protein. This multi-cell-line western blot supports detection with the JUNB Polyclonal Antibody.

Transcription Factor JunB Antibody in Human A431 Epidermoid Carcinoma Cells FACS. Flow cytometry analysis of human A431 epidermoid carcinoma cells stained with Transcription Factor JunB Antibody at 1 ug/million cells following blocking with goat serum. Cells alone are shown in red, the isotype control in green, and JUNB antibody staining in blue. The JUNB-stained population displays a clear rightward fluorescence shift relative to both controls, supporting detection of endogenous JUNB in A431 cells using this JUNB Polyclonal Antibody.