JUNB Antibody PCRP-JUNB-3G2 / JunB Monoclonal Antibody [clone PCRP-JUNB-3G2] (V9262)

JUNB Antibody PCRP-JUNB-3G2 in Human HeLa Cervical Cancer Cells IF. Immunofluorescence staining of PFA-fixed human HeLa cervical cancer cells with JUNB Antibody clone PCRP-JUNB-3G2 (green) and phalloidin counterstain (red). JUNB signal is visible primarily as fine punctate staining within the nuclei, with substantially less signal throughout the surrounding cytoplasm. Phalloidin delineates the actin cytoskeleton and cell boundaries, providing clear contrast with the predominantly nuclear JUNB distribution. This localization is consistent with the role of JunB as an AP-1 transcription factor and demonstrates cellular detection using this JunB Monoclonal Antibody.

JUNB Antibody PCRP-JUNB-3G2 in Human HeLa Cervical Cancer Cells FACS. Flow cytometry analysis of PFA-fixed human HeLa cervical cancer cells stained with JUNB Antibody clone PCRP-JUNB-3G2 (blue), compared with unstained cells (red). The antibody-stained population shows a clear rightward shift in fluorescence intensity relative to the negative control, demonstrating specific detection of endogenous JUNB in fixed HeLa cells. The well-separated fluorescence distributions support cellular detection of this AP-1 transcription factor using the JunB Monoclonal Antibody.

Purified JUNB Antibody PCRP-JUNB-3G2 SDS-PAGE. SDS-PAGE analysis of purified, BSA-free JUNB Antibody clone PCRP-JUNB-3G2 under reducing (R) and non-reducing (NR) conditions with a 2 ug antibody load. Under reducing conditions, the preparation resolves into the expected immunoglobulin heavy and light chains at approximately 50 kDa and 25 kDa, respectively. The non-reduced preparation shows a predominant intact antibody band at approximately 150 kDa. The expected electrophoretic profile provides a quality-control assessment of the integrity and purity of this JunB Monoclonal Antibody preparation.

JUNB Antibody PCRP-JUNB-3G2 Protein Microarray Validated. Protein microarray specificity testing of JUNB Antibody clone PCRP-JUNB-3G2 using a HuProt microarray containing more than 19,000 full-length human proteins. JUNB produced the highest signal with a Z-score of 132.08, compared with 20.81 for the next-ranked protein, resulting in an S-score of 111.26. The Z-score represents signal strength in standard deviations above the array mean, while the S-score represents the difference between the target and next-highest Z-scores. The exceptionally large separation of JUNB from all other proteins demonstrates strong target specificity of this JunB Monoclonal Antibody.