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Home >> Antibodies >> JLP Antibody / SPAG9 / JIP-4

JLP Antibody / SPAG9 / JIP-4 (RQ8107)

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  Catalog No Formulation Size Price (USD)  
Image RQ8107 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human A431 cells with JLP antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A431 cells with JLP antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human PC-3, 3) rat testis and 4) mouse testis tissue lysate with JLP antibody. Predicted molecular weight ~146 kDa, can be observed at ~190 kDa.
Flow cytometry testing of human HL60 cells with JLP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= JLP antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt O60271
Localization Cytoplasm
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This JLP antibody is available for research use only.
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Description

C-jun-amino-terminal kinase-interacting protein 4 (JIP-4), also called JNK-associated leucine-zipper protein (JLP), is a scaffold protein that in humans is encoded by the SPAG9 gene. This gene encodes a member of the cancer testis antigen gene family. The encoded protein functions as a scaffold protein that structurally organizes mitogen-activated protein kinases and mediates c-Jun-terminal kinase signaling. This protein also binds to kinesin-1 and may be involved in microtubule-based membrane transport. This protein may play a role in tumor growth and development. Alternate splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the JLP antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids A281-K964) was used as the immunogen for the JLP antibody.

Storage

After reconstitution, the JLP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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