J Chain Antibody / Plasma Cell and Secretory Immunoglobulin Marker Antibody (RQ8487)

J Chain Antibody U-2 OS IF. Immunofluorescence analysis of FFPE human U-2 OS cells using J Chain antibody (green) shows diffuse cytoplasmic staining consistent with intracellular localization of JCHAIN / IGJ, a secretory immunoglobulin-associated protein involved in polymeric antibody assembly, while nuclei are counterstained with DAPI (blue). HIER: steam sections in pH6 citrate buffer for 20 min.

J Chain Antibody Liver Cancer IHC. Immunohistochemistry of FFPE human liver carcinoma tissue using J Chain antibody demonstrates granular HRP-DAB brown cytoplasmic staining in scattered tumor-associated and immune-associated cell populations, consistent with JCHAIN / IGJ expression linked to secretory immunoglobulin production and plasma cell-associated immune activity. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

J Chain Antibody Thyroid Cancer IHC. Immunohistochemistry of FFPE human thyroid carcinoma tissue using J Chain antibody shows focal HRP-DAB brown cytoplasmic staining in scattered immune-associated cell populations within the tumor microenvironment, consistent with JCHAIN / IGJ expression in plasma cell-associated secretory immunoglobulin-producing cells. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

J Chain Antibody Rat Colon IHC. Immunohistochemistry of FFPE rat colon tissue using J Chain antibody demonstrates focal HRP-DAB brown cytoplasmic staining in scattered lamina propria immune cells and mucosal-associated plasma cell populations, consistent with JCHAIN / IGJ expression involved in secretory immunoglobulin production and mucosal immune defense. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

J Chain Antibody Rodent Immune Tissue WB. Western blot analysis of 1) rat small intestine and 2) mouse spleen tissue lysates using J Chain antibody detects bands at approximately 18-22 kDa, consistent with the predicted molecular weight of JCHAIN / IGJ, a secretory immunoglobulin-associated protein involved in polymeric IgA and IgM assembly. Apparent molecular weight may vary due to glycosylation and secretory immunoglobulin-associated processing.

J Chain Antibody A431 FACS. Flow cytometry analysis of fixed and permeabilized human A431 cells using J Chain antibody demonstrates a rightward shift in fluorescence intensity compared to the isotype control, consistent with intracellular detection of JCHAIN / IGJ, a secretory immunoglobulin-associated protein involved in plasma cell biology and polymeric antibody assembly; red histogram represents cells alone, green histogram represents isotype control, and blue histogram represents J Chain antibody staining.