CD104 Antibody / ITGB4 Polyclonal Antibody (RQ5890)

CD104 Antibody in Human Intestinal Cancer IF. Immunofluorescence staining of FFPE human intestinal cancer tissue with CD104 Antibody (green) and DAPI nuclear counterstain (blue). HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. The image shows green ITGB4 signal outlining portions of the epithelial structures, with prominent staining along cell and tissue boundaries surrounding the densely packed blue nuclei. This localization is consistent with the cell-matrix adhesion role of integrin beta 4 and demonstrates tissue detection using the ITGB4 Polyclonal Antibody.

CD104 Antibody in Human Rectal Cancer IHC. Immunohistochemistry staining of FFPE human rectal cancer tissue with CD104 Antibody. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. Strong brown immunoreactivity is concentrated predominantly along the epithelial glandular structures, while much of the surrounding stromal tissue shows comparatively little staining. The distribution highlights ITGB4 expression within the epithelial compartment and demonstrates detection of integrin beta 4 using the ITGB4 Polyclonal Antibody.

CD104 Antibody in Human Lung Cancer IHC. Immunohistochemistry staining of FFPE human lung cancer tissue with CD104 Antibody. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. Brown ITGB4 immunoreactivity is visible along the epithelial tumor structures, with prominent peripheral and membranous-associated staining in multiple regions of the section. This pattern is consistent with the epithelial localization of integrin beta 4 and demonstrates tissue detection using the ITGB4 Polyclonal Antibody.

CD104 Antibody Western Blot of Human Cells and Rodent Ovary. Western blot testing of human A549 (lane 1), PC-3 (lane 2), T47-D (lane 3), HeLa (lane 4), SW620 (lane 5), Caco-2 (lane 6), rat ovary (lane 7), and mouse ovary (lane 8) lysates with CD104 Antibody. Predicted molecular weight is approximately 200 kDa. A predominant high-molecular-weight band is detected near 200 kDa across the samples, with differences in signal intensity and migration between lysates. The multi-sample blot demonstrates human, rat, and mouse ITGB4 detection by the ITGB4 Polyclonal Antibody.

CD104 Antibody in Human Raji B Lymphoma Cells FACS. Flow cytometry testing of human Raji B lymphoma cells with CD104 Antibody at 1 ug/million cells following blocking with goat serum. Cells alone are shown in red, isotype control in green, and CD104 antibody staining in blue. The blue population shows a clear rightward shift relative to both the isotype control and untreated cells, demonstrating detection of cell-associated ITGB4 by the ITGB4 Polyclonal Antibody.